These GCE transgenic mice have a human glial fibrillary acidic protein (hGFAP) promoter sequence directing expression of a Cre-estrogen receptor (Cre-ERT2) fusion protein. When these transgenic mice are bred with mice containing a loxP-flanked sequence, tamoxifen-inducible Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring.
Flora Vaccarino, Yale University
Genetic Background | Generation |
---|---|
N8+pN4F2
|
Allele Type |
---|
Transgenic (Recombinase-expressing, Inducible) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
Mice hemizygous for the GCE transgene are viable and fertile, with a human glial fibrillary acidic protein (hGFAP) promoter sequence directing expression of a Cre-estrogen receptor (Cre-ERT2) fusion protein. This Cre-ERT2 fusion protein is only active when it binds to the estrogen analog 4-hydroxytamoxifen (OHT). Specifically, Cre recombinase expression is observed in subventricular zone (SVZ), dentate gyrus (DG), celebellum, mesencephalon, diencephalon, and cerebral cortex. Cre expression was also enriched in the sub-granular zone of the DG and the cerebellar Purkinje layer. When these transgenic mice are bred with mice containing a loxP-flanked sequence, tamoxifen-inducible Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring.
The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.
The GCE transgene was designed containing a Cre-estrogen receptor (Cre-ERT2) fusion protein (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain) driven by the human glial fibrillary acidic protein (hGFAP) promoter. The transgene was microinjected into fertilized (C57BL/6J x SJL/J)F2 oocytes, and founder mice from line 505 were bred to C57BL/6J mice. Subsequently these mice were backcrossed to C57BL/6J mice for at least 8 generations to establish a colony of GCE mice. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | Cre recombinase expression is observed in subventricular zone (SVZ), dentate gyrus (DG), celebellum, mesencephalon, diencephalon, and cerebral cortex. Cre expression was also enriched in the sub-granular zone of the DG and the cerebellar Purkinje layer. |
Allele Name | transgene insertion 505, Flora M Vaccarino |
---|---|
Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | GCE; Tg(GFAP-cre/ESR1)1Fmv |
Gene Symbol and Name | Tg(GFAP-cre/ERT2)505Fmv, transgene insertion 505, Flora M Vaccarino |
Gene Synonym(s) | |
Promoter | GFAP, glial fibrillary acidic protein, human |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase expression is observed in subventricular zone (SVZ), dentate gyrus (DG), celebellum, mesencephalon, diencephalon, and cerebral cortex. Cre expression was also enriched in the sub-granular zone of the DG and the cerebellar Purkinje layer. |
Strain of Origin | (C57BL/6 x SJL/J)F2 |
Chromosome | UN |
Molecular Note | The human GFAP promoter drives the inducible expression of a cre-ERT2 fusion cassette. |
Mutations Made By | Flora Vaccarino, Yale University |
When maintaining a live colony, hemizygous carriers may be bred to wildtype (noncarrier) mice from the colony.
When using the GCE mouse strain in a publication, please cite the originating article(s) and include JAX stock #012849 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(GFAP-cre/ERT2)505Fmv |
Frozen Mouse Embryo | B6.Cg-Tg(GFAP-cre/ERT2)505Fmv/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(GFAP-cre/ERT2)505Fmv/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(GFAP-cre/ERT2)505Fmv/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(GFAP-cre/ERT2)505Fmv/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.