STOCK Tg(Neurod1-cre)1Able/J

Stock number: 028364
Product name: NeuroD1-Cre
Research areas: Diabetes and Obesity Research, Research Tools

Description

These NeuroD1-Cre mice have the mouse neurogenic differentiation 1 (Neurod1) promoter driving Cre recombinase expression in Neurod1 expressing endocrine precursor cells in the pancreas, stomach and intestine.

Detailed description

The basic helix loop helix transcription factor, neurogenic differentiation 1, encoded by the Neurod1 gene, activates the insulin gene and is involved in neurosensory development and endocrine cell differentiation. These transgenic mice express Cre recombinase under the direction of the mouse Neurod1 gene promoter. When crossed to a Cre recombinase reporter strain, the reporter is detected as early as E16.5 in chromogranin A expressing endocrine cells. Cre recombinase activity is detected in insulin secreting pancreatic β cells of the fetal and adult pancreas, and in endocrine cells in the stomach and intestine. Mice that are hemizygous for the transgene are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous.

Development

The BAC clone, RPCI-23188B11, containing the entire Neurod1 gene, was modified to insert cre recombinase sequence, a nuclear localization signal, and SV40 polyadenylation sequence, into the translation initiation ATG codon of the Neurod1 gene. No other genes on the BAC were modified or deleted. DNA from a correctly modified BAC clone was used as the source for the transgenic insert microinjected into the pronuclei of fertilized oocytes of B6XB6D2F1 mice. Founder mice were subsequently established. The mice were maintained on a mixed C57BL/6 and CD1 background. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Tg(Neurod1-cre)1Able
Name: transgene insertion 1, Andrew B Leiter
MGI accession ID: MGI:5449711
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Neurod1-cre)1Able
Marker name: transgene insertion 1, Andrew B Leiter
Chromosome: UN
Strain of origin: C57BL/6 x (C57BL/6 x DBA/2)F1
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase activity is detected in insulin secreting pancreatic Β cells of the fetal and adult pancreas, and in endocrine cells in the stomach and intestine.
Molecular note: Homologous recombination introduced the sequence encoding cre recombinase into the translational initiation ATG codon of the Neurod1 gene located in a BAC. The cre sequence also included a nuclear localization signal (NLS) and the SV40 polyadenylation sequence. The purified construct was injected into pronuclei of fertilized B6xB6D2F1 oocytes. Two founders were obtained with both lines showing nearly identical patterns of cre expression. A generic line 1 was created as representative.