These MIP1-CreERT mice express a transgene with tamoxifen inducible Cre recombinase in pancreatic beta cells.
Louis H. Philipson, The University of Chicago
Genetic Background | Generation |
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N10+pN5F7
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
Starting at:
$278.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
These transgenic mice express a tamoxifen-inducible Cre recombinase (cre/ERT) driven by the mouse Ins1, insulin I, promoter. Restricted to the cytoplasm, the cre/ERT protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions of the flanked sequence. Inducible Cre recombinase activity is detected specifically in the pancreatic beta cells, and is not detected in the brain, pancreatic alpha cells, liver, fat, skeletal muscle, spleen, lungs, small intestine, large intestine, kidney, heart, eyes, testes or ovaries. No alteration of pancreatic beta cell physiology is detected. The Donating Investigator has not attempted to make the strain homozygous.
A transgenic construct containing sequence encoding cre/ERT and a 2.1-kb fragment of the human growth hormone gene, under the control of a 8.3-kb fragment of mouse insulin I promoter, was injected into fertilized CD1 mouse eggs. Founder line 1 was subsequently established. The mice were bred to (Stock No. 003474) for 10 generations by the donating lab. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony, and to remove the Gt(ROSA)26Sortm1Sor allele.
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers, on Chromosome 1, was segregating.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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Site of Expression | Inducible Cre recombinase activity is detected specifically in the pancreatic beta cells. |
Allele Name | transgene insertion 1, Louis Philipson |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | MIP-Cre/ERT; MIP-CreER; MIP-CreERT1Lphi |
Gene Symbol and Name | Tg(Ins1-cre/ERT)1Lphi, transgene insertion 1, Louis Philipson |
Gene Synonym(s) | |
Promoter | Ins1, insulin I, mouse, laboratory |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | Inducible Cre recombinase activity is detected specifically in the pancreatic beta cells. |
Strain of Origin | CD-1 |
Chromosome | UN |
Molecular Note | An 8.3 kb fragment of the mouse insulin promoter (Ins1; from nucleotide -8280 to +12 relative to the transcriptional start site) drives cDNA encoding cre recombinase fused to the mutated mouse estrogen receptor ligand binding domain. A 2.1 kb fragment of the hGH gene was added to function as a transcriptional stop signal. The 12.5 kb construct was purified and microinjected into fertilized pronuclei of CD1 mice. Line 1 was established. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make the strain homozygous.
When using the MIP-Cre/ERT mouse strain in a publication, please cite the originating article(s) and include JAX stock #024709 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Tg(Ins1-cre/ERT)1Lphi/ |
Frozen Mouse Embryo | B6.Cg-Tg(Ins1-cre/ERT)1Lphi/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Ins1-cre/ERT)1Lphi/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Ins1-cre/ERT)1Lphi/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Ins1-cre/ERT)1Lphi/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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