B6.Cg-Tg(Ins1-cre/ERT)1Lphi/J

Stock number: 024709
Product name: MIP-Cre/ERT
Research areas: Diabetes and Obesity Research, Research Tools

Description

These MIP1-CreERT mice express a transgene with tamoxifen inducible Cre recombinase in pancreatic beta cells.

Detailed description

These transgenic mice express a tamoxifen-inducible Cre recombinase (cre/ERT) driven by the mouse Ins1, insulin I, promoter. Restricted to the cytoplasm, the cre/ERT protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions of the flanked sequence. Inducible Cre recombinase activity is detected specifically in the pancreatic beta cells, and is not detected in the brain, pancreatic alpha cells, liver, fat, skeletal muscle, spleen, lungs, small intestine, large intestine, kidney, heart, eyes, testes or ovaries. No alteration of pancreatic beta cell physiology is detected. The Donating Investigator has not attempted to make the strain homozygous.

Development

A transgenic construct containing sequence encoding cre/ERT and a 2.1-kb fragment of the human growth hormone gene, under the control of a 8.3-kb fragment of mouse insulin I promoter, was injected into fertilized CD1 mouse eggs. Founder line 1 was subsequently established. The mice were bred to (Stock No. 003474) for 10 generations by the donating lab. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony, and to remove the Gt(ROSA)26Sor^tm1Sor allele.

A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers, on Chromosome 1, was segregating.

Allele

Symbol: Tg(Ins1-cre/ERT)1Lphi
Name: transgene insertion 1, Louis Philipson
MGI accession ID: MGI:4410453
Generation method: Transgenic
Attributes: Recombinase-expressing; Inducible
Marker symbol: Tg(Ins1-cre/ERT)1Lphi
Marker name: transgene insertion 1, Louis Philipson
Chromosome: UN
Strain of origin: CD-1
Expressed genes: cre/ERT2,Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of expression: Inducible Cre recombinase activity is detected specifically in the pancreatic beta cells.
Molecular note: An 8.3 kb fragment of the mouse insulin promoter (Ins1; from nucleotide -8280 to +12 relative to the transcriptional start site) drives cDNA encoding cre recombinase fused to the mutated mouse estrogen receptor ligand-binding domain. A 2.1 kb fragment of the hGH gene was added to function as a transcriptional stop signal. The 12.5 kb construct was purified and microinjected into fertilized pronuclei of CD1 mice. Line 1 was established.