Ins1CreERT2 mice express Cre-ERT2 in insulin-producing beta cells of pancreas starting at birth. They are useful for applications requiring tamoxifen-induced deletion of floxed sequences in beta cells.
Bernard Thorens, University of Lausanne
Genetic Background | Generation |
---|---|
N9+pN1F12
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Recombinase-expressing, Inducible, Null/Knockout) | Ins1 | insulin I |
Ins1CreERT2 mice express a creERT2 fusion gene from the start codon of the Ins1 (Insulin I) gene, replacing the entire coding region. Insulin is a hormone produced by beta cells in the pancreas. It regulates the metabolism of carbohydrates and fats by promoting the absorption of glucose from the blood to skeletal muscles and fat tissue and by causing fat to be stored rather than used for energy. Homozygous mice are viable and fertile. When these mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the beta cells of the offspring. The donating investigator reports that no cre expression is seen in the central nervous system.
A targeting construct was designed to insert a creERT2 fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain) at the initiation codon of the Ins1 (Insulin I) gene with followed by polyadenylation sequence and a frt-flanked neomycin resistance (neo) cassette. This construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6J mice. Mice were bred to flp transgenic mice, on a C57BL/6J background, to remove the neo cassette, and resulting mice were crossed to remove the flp-expressing transgene. Ins1 CreERT2 mice were backcrossed for at least 9 generations to C57BL/6J mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
---|---|
Site of Expression | cre/ERT2 is expressed in insulin-producing beta cells of pancreas. |
Allele Name | targeted mutation 2.1, Bernard Thorens |
---|---|
Allele Type | Targeted (Recombinase-expressing, Inducible, Null/Knockout) |
Allele Synonym(s) | Ins1CreERT2 |
Gene Symbol and Name | Ins1, insulin I |
Gene Synonym(s) | |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | cre/ERT2 is expressed in insulin-producing beta cells of pancreas. |
Strain of Origin | C57BL/6J |
Chromosome | 19 |
Molecular Note | A targeting construct was designed to insert a cre recombinase gene fused with a modified estrogen receptor gene (cre/ERT2) at the initiation codon of the gene with followed by a FRT-flanked neomycin resistance (neo) cassette, replacing the coding sequence. Flp-mediated recombination removed the FRT-flanked neo cassette. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6(Cg)-Ins1tm2.1(cre/ERT2)Thor/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026802 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Ins1<tm2.1(cre/ERT2)Thor> |
Frozen Mouse Embryo | B6(Cg)-Ins1<tm2.1(cre/ERT2)Thor>/J | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Ins1<tm2.1(cre/ERT2)Thor>/J | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Ins1<tm2.1(cre/ERT2)Thor>/J | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Ins1<tm2.1(cre/ERT2)Thor>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.