B6.Cg-Tg(Plp1-cre/ERT)3Pop/J

Stock number: 005975
Product name: PLP/creER^T
Research areas: Neurobiology Research, Research Tools

Description

These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Plp1, proteolipid protein (myelin) 1 promoter. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration allows for ablation of predetermined genes in oligodendrocytes and Schwann cells, and will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice.

Detailed description

These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Plp1, proteolipid protein (myelin) 1 promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration allows for ablation of predetermined genes in oligodendrocytes and Schwann cells, and will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Immunohistochemical analysis of brain, optic and sciatic nerve tissue reveals that the cre/Esr1 expression mimics the endogenous promoter expression pattern.

View cre expression characterization.

Development

A transgenic construct containing a 2.4 kb sequence of mouse proteolipid protein (myelin) 1 promoter, sequence encoding cre/Esr1 and SV40 polyadenylation site sequence was injected into B6D2F1 donor eggs. Founder line 3 was subsequently established. Founder animals were bred to C57BL/6J mice and then backcrossed to the same for 10 generations.

Allele

Symbol: Tg(Plp1-cre/ERT)3Pop
Name: transgene insertion 3, Brian Popko
MGI accession ID: MGI:2450391
Generation method: Transgenic
Attributes: Recombinase-expressing; Inducible
Marker symbol: Tg(Plp1-cre/ERT)3Pop
Marker name: transgene insertion 3, Brian Popko
Chromosome: UN
Strain of origin: (C57BL/6 x DBA/2)F1
Expressed genes: cre,cre recombinase,bacteriophage P1; ESR1,estrogen receptor 1,human
Site of expression: tamoxifen inducible Cre recombinase protein fused to mutant form of the mouse estrogen receptor ligand binding domain; expression in oligodendrocytes and Schwann cells
Molecular note: The transgenic construct encodes a tamoxifen-inducible fusion protein consisting of cre recombinase and a mutated human estrogen receptor ligand-binding domain cloned next to a Plp promoter. The 2.5 kb Plp regulatory region includes 5' flanking DNA, exon 1, and intron 1. Transgene expression was confirmed by RT-PCR and Western blot analysis. The mouse Plp promoter was used to drive transgene expression in myelinating cells.