These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Plp1, proteolipid protein (myelin) 1 promoter. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration allows for ablation of predetermined genes in oligodendrocytes and Schwann cells, and will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice.
Brian Popko, University of Chicago
Genetic Background | Generation |
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N10+N3F9
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Plp1, proteolipid protein (myelin) 1 promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration allows for ablation of predetermined genes in oligodendrocytes and Schwann cells, and will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Immunohistochemical analysis of brain, optic and sciatic nerve tissue reveals that the cre/Esr1 expression mimics the endogenous promoter expression pattern.
View cre expression characterization.
A transgenic construct containing a 2.4 kb sequence of mouse proteolipid protein (myelin) 1 promoter, sequence encoding cre/Esr1 and SV40 polyadenylation site sequence was injected into B6D2F1 donor eggs. Founder line 3 was subsequently established. Founder animals were bred to C57BL/6J mice and then backcrossed to the same for 10 generations.
Expressed Gene | ESR1, estrogen receptor 1, human |
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Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | tamoxifen inducible Cre recombinase protein fused to mutant form of the mouse estrogen receptor ligand binding domain; expression in oligodendrocytes and Schwann cells |
Allele Name | transgene insertion 3, Brian Popko |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | PLP/CreER; Plp-CreERt; Plp-Cre-ERT2; Tg(Plp1-cre/ESR1)3.16Pop; Tg(Plp1-cre/ESR1)3Pop; Tg(Plp-cre/ESR1)3.16Pop |
Gene Symbol and Name | Tg(Plp1-cre/ERT)3Pop, transgene insertion 3, Brian Popko |
Gene Synonym(s) | |
Promoter | Plp1, proteolipid protein (myelin) 1, mouse, laboratory |
Expressed Gene | ESR1, estrogen receptor 1, human |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | tamoxifen inducible Cre recombinase protein fused to mutant form of the mouse estrogen receptor ligand binding domain; expression in oligodendrocytes and Schwann cells |
Strain of Origin | (C57BL/6 x DBA/2)F1 |
Chromosome | UN |
Molecular Note | The transgenic construct encodes a tamoxifen inducible fusion protein consisting of cre recombinase and a mutated human estrogen receptor ligand binding domain cloned next to a Plp promoter. The 2.5 kb Plp regulatory region includes 5' flanking DNA, exon 1, and intron 1. Transgene expression was confirmed by RT-PCR and Western blot analysis. The mouse Plp promoter was used to drive transgene expression in myelinating cells. |
When maintaining a live colony, these mice are bred as hemizygotes.
When using the PLP/creERT mouse strain in a publication, please cite the originating article(s) and include JAX stock #005975 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Plp1-cre/ESR1)3.16Pop |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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