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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR1544 | CAC GTG GGC TCC AGC ATT | Internal Positive Control Forward | A | |||
oIMR3580 | TCA CCA GTC ATT TCT GCC TTT G | Internal Positive Control Reverse | A | |||
oIMR8940 | AGT AGT GAT CAG GTA TTG CTG TTA TCT G | Forward | A | |||
oIMR8941 | GAC TAA TGT TGT GGG AAA TTG GAG | Reverse | A | |||
TmoIMR0105 | Fluorophore-1 | CCA ATG GTC GGG CAC TGC TCA A | Quencher-1 | IC Probe | A | |
TmoIMR0126 | Fluorophore-2 | TAT ACG TTG TCC TGG CCA CGG CA | Quencher-2 | Tg Probe | A |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
oIMR1544 | 0.40 uM |
oIMR3580 | 0.40 uM |
oIMR8940 | 0.40 uM |
oIMR8941 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
029290 | B6(Cg)-Sgtatm1.1Drmcr/J |
021072 | B6(Cg)-Slc6a8tm1.2Clar/J |
021828 | B6(SJL)-Lrrk2tm3.1Mjff/J |
018445 | B6.129-Mre11atm1.1Rchd/J |
022364 | B6.129P2(SJL)-Ntrk3tm1Ddg/J |
013085 | B6.129S-Pbx1tm3.1Mlc/J |
018540 | B6.129S6(Cg)-Bpnt1tm2.1Yrk/J |
034712 | B6.Cg-Cyp27a1tm1.1Dpm/J |
024469 | B6.Cg-Lrrk2tm3.1Mjfa/J |
021191 | B6.Cg-Pvalbtm2.1(flpe)Hze/J |
013717 | B6.Cg-Slc20a1tm1.2Cmg/J |
012627 | B6.Cg-Stard4tm1.1Bres/J |
012631 | B6.Cg-Tyrc-2J Sharpintm1.1Sun/J |
005703 | B6.Cg-Tg(ACTFLPe)9205Dym/J |
036551 | B6.FVB(Cg)-Tg(Pmch-cre)1Lowl/J |
012653 | B6;129-Pax7tm1.1Fan/J |
014168 | B6;129-Pot1atm1.1Tdl/J |
021443 | B6;129S-Tgif1tm1Caw/J |
012623 | B6;129S4-Carftm1.1Aew/J |
016168 | B6;129S4-Fbxl3Gt(FHCRC-GT-S13-12G1)Sor/JtJ |
018353 | B6;129S4-Ror1tm1.1Meg/J |
022407 | B6;D2-Tg(Phox2b-flpo)3276Grds/J |
026526 | B6;SJL-Akap6tm1.1Mskf/Mmjax |
034731 | B6N(129S4)-Srd5a3tm1c(EUCOMM)Wtsi/J |
022365 | C57BL/6-Il15ratm2.1Ama/J |
035185 | STOCK Adgrl1tm2c(EUCOMM)Hmgu/SudJ |
025665 | STOCK Asxl1tm1.1Iaai/J |
023199 | STOCK Brsk2tm2.1Jrs/J |
026199 | STOCK F8tm1.1Rmnt/J |
010702 | STOCK Gad2tm1(cre/ERT2)Zjh/J |
022972 | STOCK Pros1tm1Grl/J |
014099 | STOCK Tg(Pmch-cre)1Lowl/J |
32 strains use this protocol |