These transgenic mice have the murine pro-melanin-concentrating hormone (Pmch or MCH) promoter/enhancer regions within the BAC transgene directing expression of Cre-recombinase. These mice may be useful for studying MCH-neuronal activities in controlling energy balance, glucose homeostasis, sleep, locomotion, and reward-related behaviors.
Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Recombinase-expressing) |
Pmch-cre (or MCH-Cre) transgenic mice have the murine pro-melanin-concentrating hormone (Pmch) promoter/enhancer regions within the BAC transgene directing expression of Cre-recombinase. Hemizygous Pmch-cre mice are viable, fertile, and normal in size, with cre expression directed specifically to MCH-neurons of the hypothalamus and zona incerta. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing neurons of the offspring. MCH neurons regulate many physiological functions by projecting through the brain. These mice may be useful for studying MCH-neuronal activities in controlling energy balance, glucose homeostasis, sleep, locomotion, and reward-related behaviors.
A murine bacterial artificial chromosome (BAC), containing the pro-melanin-concentrating hormone (Mch), was modified by targeting a Cre-recombinase sequence and frt-flanked neomycin resistance (neo) cassette into the translational start site of the Mch locus. The targeted BAC sequences were further modified using FLP recombination to remove the neo cassette. This modified BAC transgene was microinjected into the pronuclei of fertilized FVB/NJ oocytes. Transgenic mice were bred to B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J mice (Stock No. 004718) to establish a colony of Mch-cre mice. These mice were subsequently maintained by breeding with mice on a C57BL/6 x 129 mixed background. These mice no longer contain the (CAG-Bgeo/GFP) transgene. Upon arrival at The Jackson Laboratory arrival, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | cre expression is directed specifically to the melanin-concentrating hormone (MCH) neurons of the hypothalamus and zona incerta. |
Allele Name | transgene insertion 1, Bradford B Lowell |
---|---|
Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | MCH-Cre |
Gene Symbol and Name | Tg(Pmch-cre)1Lowl, transgene insertion 1, Bradford B Lowell |
Gene Synonym(s) | |
Promoter | Pmch, pro-melanin-concentrating hormone, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | cre expression is directed specifically to the melanin-concentrating hormone (MCH) neurons of the hypothalamus and zona incerta. |
Strain of Origin | FVB/NJ |
Chromosome | UN |
Molecular Note | Homologous recombination with EL250 cells was induced to insert a cre-polyA-FRT-Neo-FRT cassette into a BAC containing the Pmch (pro-melanin-concentrating hormone; MCH) at the Pmch translational start site with concomitant replacement of the 26 nucleotides following it. Arabinose-induced Flp recombination in bacterial cells removed the kanamycin cassette. The purified, linearized construct was injected into the pronuclei of FVB one-cell embryos. Eight lines were generated and crossed to Z/EG reporter animals. 2 lines exhibited GFP expression in the lateral hypothalamus and zona incerta, known sites of endogenous Pmch expression. Cre expression in one of these lines of characterized further. |
Mutations Made By | Bradford Lowell, Beth Israel Deaconess Med Cntr (Harvard) |
When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) mice from the colony or to FVB/NJ inbred mice (Stock No. 001800). The donating investigator reports they have not tried to generate homozygous mice.
When using the Mch-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #014099 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(Pmch-cre)1Lowl |
Frozen Mouse Embryo | STOCK Tg(Pmch-cre)1Lowl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Pmch-cre)1Lowl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Pmch-cre)1Lowl/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Pmch-cre)1Lowl/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.