These floxed Bpnt1 mutant mice possess loxP sites flanking exons 4- 5 and are useful in generating tissue-specific conditional mutations.
John York, Duke University Medical Center
Bpnt1 is (bisphosphate 3'-nucleotidase 1) is a magnesium-dependent phosphomonoesterase that can be inhibited by lithium, a drug used to treat manic depression.
Exons 4 and 5 are flanked by loxP sites in these Bpnt1 conditionally targeted mutation mice. The floxed mice do not show any over phenotype and expression of BPNT1 is normal with no detectable defects in liver, kidney, brain and small intestine. When the floxed segment is excised by Cre recombinase, tissue-specific knockouts of the gene can be produced.
A loxP site was introduced to intron 3 and FRT-neomycin-FRT-loxP sequences were added to intron 5 of the targeted gene. 129S6/SvEvTac-derived embryonic stem (ES) cells were used to create the mutation. The neomycin cassette was excised by crossing F2 mice to B6.Cg-Tg(ACTFLPe)9205Dym/J animals that express FLP recombinase under the control of the actin promoter (see Stock No. 005703). Resultant mice were backcrossed eight times to C57BL/6 by the donating laboratory.
|Allele Name||targeted mutation 2.1, John D York|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Bpnt1, bisphosphate 3'-nucleotidase 1|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A loxP site was introduced to intron 3 and FRT-neomycin-FRT-loxP sequences were added to intron 5 of the targeted gene. Flp-mediated recombination removed the neo cassette.|
Homozygous and heterozygous floxed mice are viable and fertile.
When using the B6.129S6(Cg)-Bpnt1tm2.1Yrk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018540 in your Materials and Methods section.