These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).
For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase in myogenic progenitor cells (see Stock No. 012476), this mutant mouse strain may be useful in studies muscle stem cells.
A PGK-Neo selection cassette flanked by FRT sites was inserted downstream of exon 2 of the targeted gene. A loxP site was inserted downstream of the selection cassette. Another loxP site was inserted upstream of exon 2. The targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+ derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to mice expressing FLP recombinase under the control of an Actin promoter (on the C57BL/6 background) to remove the neo selection cassette. The mice were then intercrossed for 4 generations. Upon arrival at The Jackson Laboratory the mice were crossed to C57BL/6J once to establish the colony.
|Allele Name||targeted mutation 1.1, Chen-Ming Fan|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Pax7f; Pax7loxP-Le|
|Gene Symbol and Name||Pax7, paired box 7|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A loxP site was inserted upstream of exon 2 and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 2. The neo cassette was removed by flp mediated recombination leaving exon 2 floxed.|
|Mutations Made By|| |
Chen-Ming Fan, Carnegie Institution of Washington
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129-Pax7tm1.1Fan/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012653 in your Materials and Methods section.