C57BL/6-Il15ra^tm2.1Ama/J

Stock number: 022365
Product name: Il15ra^fl
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These IL15ra^fl mice possess loxP sites flanking exons 2-3 of the interleukin 15 receptor, alpha chain (Il15ra) gene. This strain may be useful for studying the function of IL-15Rα when presented on different types of lymphocytes.

Detailed description

These Il15ra^fl mice possess loxP sites flanking exons 2-3 of the interleukin 15 receptor, alpha chain (Il15ra) gene. IL-15Rα presents mainly on dendritic cells, monocytes and macrophages, and plays a role in the induction of memory T cell proliferation, and Natural Killer (NK) cell survival and cell activation. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 2-3 deleted in the cre-expressing tissues.

Development

A targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette followed by a loxP site upstream of exon 2, and a second loxP site downstream of exon 3 of the interleukin 15 receptor, alpha chain (Il15ra) gene. The construct was electroporated into C57BL/6J-derived PRX-B6T embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Flpe expression plasmid to delete the neo cassette. Resulting ES cells, containing the floxed-exons, were injected into blastocysts and progeny were crossed to remove the Flp-expressing transgene (see SNP note below). Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57Bl6J;C57BL/6N genetic background.

Allele

Symbol: Il15ra^tm2.1Ama
Name: targeted mutation 2.1, Averil Ma
MGI accession ID: MGI:4417841
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Il15ra
Marker name: interleukin 15 receptor, alpha chain
Chromosome: 2
Strain of origin: C57BL/6J
Molecular note: LoxP sites flanking exons 2 and 3 as well as an FRT flanked neomycin expression cassette were inserted by homologous recombination. The neomycin resistance cassette was then removed by flp mediated recombination.