These IL15rafl mice possess loxP sites flanking exons 2-3 of the interleukin 15 receptor, alpha chain (Il15ra) gene. This strain may be useful for studying the function of IL-15Rα when presented on different types of lymphocytes.
Averil Ma, University of California, San Francisco
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Il15ra | interleukin 15 receptor, alpha chain |
These Il15rafl mice possess loxP sites flanking exons 2-3 of the interleukin 15 receptor, alpha chain (Il15ra) gene. IL-15Rα presents mainly on dendritic cells, monocytes and macrophages, and plays a role in the induction of memory T cell proliferation, and Natural Killer (NK) cell survival and cell activation. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 2-3 deleted in the cre-expressing tissues.
A targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette followed by a loxP site upstream of exon 2, and a second loxP site downstream of exon 3 of the interleukin 15 receptor, alpha chain (Il15ra) gene. The construct was electroporated into C57BL/6J-derived PRX-B6T embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Flpe expression plasmid to delete the neo cassette. Resulting ES cells, containing the floxed-exons, were injected into blastocysts and progeny were crossed to remove the Flp-expressing transgene (see SNP note below). Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57Bl6J;C57BL/6N genetic background.
Allele Name | targeted mutation 2.1, Averil Ma |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Il15rafl |
Gene Symbol and Name | Il15ra, interleukin 15 receptor, alpha chain |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 2 |
Molecular Note | LoxP sites flanking exons 2 and 3 as well as an FRT flanked neomycin expression cassette were inserted by homologous recombination. The neomycin resistance cassette was then removed by flp mediated recombination. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Il15rafl mouse strain in a publication, please cite the originating article(s) and include JAX stock #022365 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Il15ra<tm2.1Ama> |
Frozen Mouse Embryo | C57BL/6-Il15ra<tm2.1Ama>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Il15ra<tm2.1Ama>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Il15ra<tm2.1Ama>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Il15ra<tm2.1Ama>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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