ACTBFLPe mice are useful for deletion of Frt-flanked genes in a variety of tissues including spinal cord, heart, gonad, and adrenal gland.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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N11F13
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Allele Type |
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Transgenic (Recombinase-expressing) |
This transgenic strain expresses a variant of the Saccharomyces cerevisiae FLP1 recombinase gene under the direction of the human ACTB promoter. The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being four-fold and ten-fold that of wildtype FLP at 37oC and 40oC, respectively. Mice that are hemizygous for the transgenic allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5. This deleter strain is a suitable alternative to, and complement with the Cre-loxP system for in vivo genetic engineering.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct containing a FLP1 recombinase variant controlled by the human ACTB promoter (3 kb flanking sequence, 78-bp 5' untranslated region, 832-bp of exon 1) was injected into fertilized eggs from B6SJLF2 mice. Founder animals were obtained and crossed with C57BL/6J mice for at least 5 generations.
Expressed Gene | FLP, FLP recombinase, Saccharomyces cerevisiae |
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Site of Expression | expression of the enhanced FLP1 recombinase variant (FLPe) is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5 |
Allele Name | transgene insertion 9205, Susan Dymecki |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | ACTB::FLPe; ACTB:FLPe; ACTB-Flpe; ACTFlpe; ACT-FLPe; actin-FLPe; beta-actinFLPe; CAGG-Flpr; CAGGS-flp; Flp; Flp deleter; FLPe; hACTB::FLPe; hACTB-FLPe; Tg (ACTFLEe)9205Dym; Tg(ACTB-FlpE)9205Dym; TgaFlpe; TgpCAG-flpe |
Gene Symbol and Name | Tg(ACTFLPe)9205Dym, transgene insertion 9205, Susan Dymecki |
Gene Synonym(s) | |
Promoter | ACTB, actin beta, human |
Expressed Gene | FLP, FLP recombinase, Saccharomyces cerevisiae |
Site of Expression | expression of the enhanced FLP1 recombinase variant (FLPe) is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5 |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
General Note | The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being 4X and 10X that of wildtype FLP at 37°C and 40°C, respectively. Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. |
Molecular Note | Sequence encoding FLP1 was adjoined to human ACTB promoter sequence, including 3 kb of promoter sequence and an 0.8 kb portion of exon 1. The promoter region drives expression in the germline. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5. |
Mutations Made By | Susan Dymecki, Harvard Medical School |
When maintaining a live colony, these mice may be bred as homozygotes. Coat color expected from breeding is Black.
When using the ACTB:FLPe B6J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005703 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(ACTFLPe)9205Dym |
Frozen Mouse Embryo | B6.Cg-Tg(ACTFLPe)9205Dym/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(ACTFLPe)9205Dym/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(ACTFLPe)9205Dym/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(ACTFLPe)9205Dym/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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