B6.Cg-Tg(ACTFLPe)9205Dym/J

Stock number: 005703
Product name: ACTB:FLPe B6J
Research areas: Research Tools

Description

ACTBFLPe mice are useful for deletion of Frt-flanked genes in a variety of tissues including spinal cord, heart, gonad, and adrenal gland.

Detailed description

This transgenic strain expresses a variant of the Saccharomyces cerevisiae FLP1 recombinase gene under the direction of the human ACTB promoter. The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being four-fold and ten-fold that of wildtype FLP at 37^oC and 40^oC, respectively. Mice that are hemizygous for the transgenic allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5. This deleter strain is a suitable alternative to, and complement with the Cre-loxP system for in vivo genetic engineering.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A transgenic construct containing a FLP1 recombinase variant controlled by the human ACTB promoter (3 kb flanking sequence, 78-bp 5' untranslated region, 832-bp of exon 1) was injected into fertilized eggs from B6SJLF2 mice. Founder animals were obtained and crossed with C57BL/6J mice for at least 5 generations.

Allele

Symbol: Tg(ACTFLPe)9205Dym
Name: transgene insertion 9205, Susan Dymecki
MGI accession ID: MGI:2448985
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(ACTFLPe)9205Dym
Marker name: transgene insertion 9205, Susan Dymecki
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: FLP,FLP recombinase,Saccharomyces cerevisiae
Site of expression: expression of the enhanced FLP1 recombinase variant (FLPe) is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5
Molecular note: Sequence encoding FLP1 was adjoined to human ACTB promoter sequence, including 3 kb of promoter sequence and an 0.8 kb portion of exon 1. The promoter region drives expression in the germline. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5.
General note: The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being 4X and 10X that of wildtype FLP at 37°C and 40°C, respectively. Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.