| Allele Name | transgene insertion 9205, Susan Dymecki |
| Allele Type | Transgenic (Recombinase-expressing) |
| Allele Synonym(s) | ACTB::FLPe; ACTB:FLPe; ACTB-Flpe; ACTFlpe; ACT-FLPe; actin-FLPe; beta-actinFLPe; CAGG-Flpr; CAGGS-flp; Flp; Flp deleter; FLPe; hACTB::FLPe; hACTB-FLPe; Tg (ACTFLEe)9205Dym; Tg(ACTB-FlpE)9205Dym; TgaFlpe; TgpCAG-flpe |
| Gene Symbol and Name | Tg(ACTFLPe)9205Dym, transgene insertion 9205, Susan Dymecki |
| Gene Synonym(s) | |
| Promoter | ACTB, actin beta, human |
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| Expressed Gene | FLP, FLP recombinase, Saccharomyces cerevisiae |
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| Site of Expression | expression of the enhanced FLP1 recombinase variant (FLPe) is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5 |
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| Strain of Origin | (C57BL/6 x SJL)F2 |
| Chromosome | UN |
| General Note | The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being 4X and 10X that of wildtype FLP at 37°C and 40°C, respectively. Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. |
| Molecular Note | Sequence encoding FLP1 was adjoined to human ACTB promoter sequence, including 3 kb of promoter sequence and an 0.8 kb portion of exon 1. The promoter region drives expression in the germline. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5. |
| Mutations Made By | Susan Dymecki, Harvard Medical School |
