These Pbx1f/f mutant mice possess loxP sites flanking exon 3 of the targeted pre B-cell leukemia transcription factor 1 (Pbx1) gene. This strain may be useful for studying the effect of Pbx1 on the maintenance and development of multiple tissue lineages.
Michael L Cleary, Stanford University School of Medicine
These Pbx1f/f mutant mice possess loxP sites flanking exon 3 of the targeted pre B-cell leukemia transcription factor 1 (Pbx1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues. This strain may be useful for studying the effect of Pbx1 on the maintenance and development of multiple tissue lineages.
For example, when crossed to a strain expressing interferon-inducible Cre recombinase in multiple tissues (see Stock No. 003556), this mutant mouse strain may be useful in studies of hematopoietic stem cell renewal.
When crossed to a strain expressing Cre recombinase in endothelial cells (see Stock No. 008863), this mutant mouse strain may be useful in studies of studies of hematopoietic stem cell renewal.
A targeting vector was designed to insert a loxP site upstream of exon 3, and a second loxP site downstream of exon 3 of the pre B-cell leukemia transcription factor 1 (Pbx1) gene. The floxed exon was followed by a frt-flanked resistance cassette. The construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. Offspring, heterozygous for this Pbx1 allele, were bred with
129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym transgenic mice to delete the resistance cassette. Progeny were crossed to remove the Flp-expressing mutation, resulting in a colony homozygous for the floxed-Pbx1 (Pbx1f/f) allele. These mice were backcrossed to C57BL/6J mice for at least 14 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664 for at least one generation to establish the colony.
|Allele Name||targeted mutation 3.1, Michael L Cleary|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Pbx1, pre B cell leukemia homeobox 1|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A loxP site was inserted upstream of exon 3 and a loxP site and an frt flanked selection cassette were inserted downstream of exon 3 via homologous recombination. The selection cassette was then removed by Flp mediated recombination leaving behind a single frt site.|
|Mutations Made By|| |
Michael Cleary, Stanford University School of Medicine
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129S-Pbx1tm3.1Mlc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013085 in your Materials and Methods section.