When bred to a strain expressing Cre recombinase throughout all tissues, this floxed mutant mouse strain may be useful in studies of intracellular cholesterol trafficking.
Dr. Jan L. Breslow, Rockefeller University
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in cre-expressing tissues.
A targeting vector containing an FRT site flanked NEO cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 3 of the targeted gene, and loxP sites were placed flanking exon 3. The construct was electroporated into B6(Cg)-Tyrc-2J-derived CY2.4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to transgenic mice expressing FLPe recombinase under the control of an actin promoter, B6.Cg-Tg(ACTFLPe)9205Dym/J (Stock No. 005703). Mice that retained the loxP site flanked exon 3 were then bred to C57BL/6J mice to remove the FLPe transgene. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory the mice were crossed to C57BL/6J at least once to establish the colony.
|Allele Name||targeted mutation 1.2, Jan L Breslow|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Stard4, StAR-related lipid transfer (START) domain containing 4|
|Strain of Origin||B6(Cg)-Tyrc-2J|
|Molecular Note||Cre mediated recombination of Stard4tm1.1Bres removed exon 3. The absence of protein expression was confirmed by western blot analysis on liver extracts.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.Cg-Stard4tm1.1Bres/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012627 in your Materials and Methods section.
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