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Technical Information Scientists
Notes
27410 expected CAG size is between 180 and 200
For additional information please contact Laragen directly:
Jin Li
Laragen, Inc.
10601 Virginia Ave.
Culver City, CA 90232
310 280 0804
The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Expected Results
JAX Protocol
Protocol Primers
| Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
|---|
Strains Using This Protocol
| Stock Number | Strain Name |
|---|---|
| 039888 | B6(129P2)-Ddx3xem1Tdk/J |
| 037071 | B6.129-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh Is(14)2Rdf/CjwuJ |
| 003598 | B6.129-Htttm5Mem/J |
| 037206 | B6.129-Optntm1.1Htse/J |
| 004595 | B6.129P2-Htttm2Detl/150J |
| 021193 | B6.129P2-Htttm2Detl/365J |
| 037673 | B6.129S-Atxn1em3Hzo/J |
| 027411 | B6.129S1(Cg)-Htttm2Mem/20ChdiJ |
| 036156 | B6.129S1-Httem2(HTT)Chdi/J |
| 029928 | B6.129S1-Htttm1.1Mfc/190ChdiJ |
| 036157 | B6.129S1-Htttm8(HTT*)Szi Httem3(HTT)Chdi/J |
| 037918 | B6.129S1-Htttm8(HTT*)Szi/Mmjax |
| 027412 | B6.129S4-Htttm1.1Pfs/80ChdiJ |
| 007218 | B6.129S6-Trp53tm2Xu/J |
| 005601 | B6.129S7-Atxn1tm1Hzo/J |
| 036101 | B6.Cg-Atxn1em2Hzo/J |
| 012329 | B6.Cg-Fxntm1.1Pand Fxntm1Mkn/J |
| 014162 | B6.Cg-Fxntm1Mkn Fxntm1Pand/J |
| 012910 | B6.Cg-Fxntm1Mkn Tg(FXN)YG22Pook/J |
| 012253 | B6.Cg-Fxntm1Mkn Tg(FXN)YG8Pook/J |
| 027430 | B6.Cg-Gt(ROSA)26Sortm47(HTT)Arte/ChdiJ |
| 027429 | B6.Cg-Gt(ROSA)26Sortm48(HTT)Arte/ChdiJ |
| 027428 | B6.Cg-Gt(ROSA)26Sortm49(HTT)Arte/ChdiJ |
| 027427 | B6.Cg-Gt(ROSA)26Sortm50(HTT)Arte/ChdiJ |
| 027426 | B6.Cg-Gt(ROSA)26Sortm51(HTT)Arte/ChdiJ |
| 027431 | B6.Cg-Gt(ROSA)26Sortm52(HTT)Arte/ChdiJ |
| 027435 | B6.Cg-Htttm1(HTT)Arte/ChdiJ |
| 036155 | B6.Cg-Htttm1(HTT*)Chdi Httem1(HTT)Chdi/J |
| 007001 | B6.Cg-Ndor1Tg(UBC-cre/ERT2)1Ejb/1J |
| 008085 | B6.Cg-Ndor1Tg(UBC-cre/ERT2)1Ejb/2J |
| 040737 | B6.Cg-Ptprca Pepcb Gt(ROSA)26Sortm1.1(CAG-cas9*,-EGFP)Fezh Tg(TcraTcrb)425Cbn/ShrJ |
| 040736 | B6.Cg-Ptprca Pepcb Gt(ROSA)26Sortm1.1(CAG-cas9*,-EGFP)Fezh Tg(TcrLCMV)327Sdz/ShrJ |
| 040288 | B6.Cg-Ptprca Pepcb Tg(TcraTcrb)1100Mjb Gt(ROSA)26Sortm1.1(CAG-cas9*,-EGFP)Fezh/ShrJ |
| 029721 | B6.Cg-Pvalbtm1(cre)Arbr Fxnem2Lutzy Fxnem2.1Lutzy/J |
| 039983 | B6.Cg-Tg(AR*121Q)3Als/J |
| 029436 | B6.Cg-Tg(CMV-AR*113Q)FMonk/J |
| 008586 | B6.Cg-Tg(FXN*)1Sars Fxntm1Mkn/J |
| 006471 | B6.Cg-Tg(HDexon1)61Gpb/J |
| 007051 | B6.Cg-Tg(tetO-APPSwInd)102Dbo/Mmjax |
| 027432 | B6.FVB-Tg(YAC128)53Hay/ChdiJ |
| 012705 | B6;CBA-Tg(ATXN3*)84.2Cce/IbezJ |
| 003627 | B6C3-Tg(HD82Gln)81Gschi/J |
| 002810 | B6CBA-Tg(HDexon1)62Gpb/1J |
| 006494 | B6CBA-Tg(HDexon1)62Gpb/3J |
| 026175 | B6J.129(B6N)-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh/J |
| 026179 | B6J.129(Cg)-Gt(ROSA)26Sortm1.1(CAG-cas9*,-EGFP)Fezh/J |
| 027409 | B6J.129S1-Htttm1Mfc/140ChdiJ |
| 027410 | B6J.129S1-Htttm1Mfc/190ChdiJ |
| 370476 | B6J.129S1-Htttm1Mfc/190JChdi |
| 027419 | B6J.Cg-Tg(HDexon1)62Gpb/110ChdiJ |
| 027421 | B6J.Cg-Tg(HDexon1)62Gpb/160ChdiJ |
| 038997 | B6N.Cg-Hprt1tm1(Gdf9-cas9)Jahe/J |
| 027413 | B6N.Cg-Htttm1Xen/45ChdiJ |
| 027414 | B6N.Cg-Htttm2Xen/45ChdiJ |
| 027415 | B6N.Cg-Htttm3Xen/80ChdiJ |
| 027416 | B6N.Cg-Htttm4Xen/80ChdiJ |
| 027417 | B6N.Cg-Htttm5Xen/105ChdiJ |
| 027418 | B6N.Cg-Htttm6Xen/105ChdiJ |
| 037451 | C57BL/6-Ifitm3em1Jsy/J |
| 023088 | C57BL/6J-Tg(C9orf72_i2)8Lutzy/J |
| 023099 | C57BL/6J-Tg(C9orf72_i3)112Lutzy/J |
| 003651 | C57BL/6J-Tg(Zp3-cre)93Knw/J |
| 027422 | D2.Cg-Tg(HDexon1)62Gpb/160ChdiJ |
| 027424 | D2.Cg-Tg(HDexon1)62Gpb/240ChdiJ |
| 027425 | D2.Cg-Tg(HDexon1)62Gpb/280ChdiJ |
| 004938 | FVB-Tg(YAC128)53Hay/J |
| 038293 | FVB.Cg-Pde6b+ Tyrc-ch Ccdc198em1Iad/J |
| 032031 | FVB/N-Tg(HSA*LR)20bCath/J |
| 027379 | FVB/N-Tg(HTT*)NXwy/J |
| 008197 | FVB/N-Tg(HTT*97Q)IXwy/J |
| 027433 | FVB/N-Tg(HTT*97Q)LXwy/ChdiJ |
| 036633 | FVB/NJ-Tg(C9orf72_i3)101Lutzy/J |
| 037050 | FVB/NJ-Tg(HTT*131Q)BACXwy/120J |
| 007850 | J:NU |
| 035970 | NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(YAC128)53Hay/JnolJ |
| 026014 | NOD.Cg-Rag1tm1Mom KitW-41J Il2rgtm1Wjl/EavJ |
| 017914 | NOD.Cg-Rag1tm1Mom Il2rgtm1Wjl Tg(HLA-DRA,HLA-DRB1*0401)39-2Kito/ScasJ |
| 007799 | NOD.Cg-Rag1tm1Mom Il2rgtm1Wjl/SzJ |
| 014568 | NOD.Cg-Rag1tm1Mom Ins2Akita Il2rgtm1Wjl/SzJ |
| 003118 | STOCK Ces1ce Foxn1nu/J |
| 031007 | STOCK Fxnem2.1Lutzy Tg(FXN)Y47Pook/J |
| 030395 | STOCK Fxnem2.1Lutzy Tg(FXN)YG8Pook/800J |
| 030930 | STOCK Fxnem2.1Lutzy Tg(FXN)YG8Pook/J |
| 024097 | STOCK Fxntm1Mkn Tg(FXN)Y47Pook/J |
| 024113 | STOCK Fxntm1Mkn Tg(FXN)YG8Pook/2J |
| 010963 | STOCK Fxntm1Mkn Tg(FXN)YG22Pook/J |
| 008398 | STOCK Fxntm1Mkn Tg(FXN)YG8Pook/J |
| 003456 | STOCK Htttm5Mem/J |
| 013169 | STOCK Nphp1tm1Jgg/J |
| 006770 | STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J |
| 035561 | STOCK Tc(HSA21,CAG-EGFP)1Yakaz/J |
| 030324 | STOCK Tg(FXN)YG8Pook/J |
| 018299 | STOCK Tg(FXN*)1Sars Fxntm1Mkn/J |
| 93 strains use this protocol | |