Humanized NSG™ (hu-NSG™) mouse models are powerful tools for studying cancer, inflammatory and infectious disease, and hematopoiesis. We maintain large on-the-shelf inventories of two versatile humanized models.
Humanized CD34+ mice are supreme in vivo models to study immuno-oncology, infectious diseases and graft rejection research. This model has the longest research span, over 12 months with a functional human immune system and displays T-cell dependent inflammatory responses, with no donor cell immune reactivity towards host. Hu-CD34+ mice are produced by injecting CD34+ and yield robust multilineage immune systems with good T cell maturation and function for long-term studies.
Humanized PBMC mice are used as in vivo models to study and evaluate compounds for infectious diseases and graft rejection research. This model has the fastest engraftment rate using adult peripheral blood mononuclear cells and enables short-term studies requiring a strong effector and memory T cell function.
We utilize the NOD scid gamma (NSG™) mouse, developed by JAX Professor Lenny Shultz, as the host. NSG™ mice have been shown to support greater engraftment of human hematopoietic stem cells (hu-CD34+ cells) than all other strains (McDermott et al. 2010). Our suite of humanized NSG™ strains, with additional modifications of host genes and tissues, offer an enhanced ability to accurately recapitulate specific functions of human cells in vivo.
Humanized mouse models, where human tumor tissue is engrafted into immunodeficient NOD SCID gamma (NSG) mice, are frequently used in oncology and infectious disease studies to provide valuable translational insights.