Diabetes onset was monitored weekly between the ages of 3 and 30 weeks in 120 female and 80 male NOD/ShiLtJ mice. Mice were fed a 6% fat diet (LabDiet® 5K52 formulation), ad libitum. Diabetes was defined by a non-fasting blood glucose level exceeding 250 mg/dL. Blood was sampled in the morning from the submental route and analyzed using a OneTouch Ultra 2 handheld glucometer that was validated using a control solution on each measurement day.
Median female onset was 18 weeks. Data were combined from two independent studies conducted in a standard barrier room in Bar Harbor, ME, and a maximum barrier room in Sacramento, CA; each included 60 females and 40 males. The Kaplan-Meier onset curves from the individual studies did not differ significantly for females or males (log-rank P values of 0.25 and 0.99, respectively).
This study was conducted in 2025. Median female onset was the same in studies conducted in 2022 and 2017. The onset curve from 2025 did not differ significantly from either prior study (P=0.96 and 0.44, respectively).
Body weights from this study are also available.
