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FAM = Mut
CY5 = IPC
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR1544 | CAC GTG GGC TCC AGC ATT | Internal Positive Control Forward | A | |||
oIMR3580 | TCA CCA GTC ATT TCT GCC TTT G | Internal Positive Control Reverse | A | |||
oIMR5260 | TGG GTT CAA ACA AAG GTG CAA | Transgene Forward | A | |||
oIMR7707 | GAT GAC GAT CAC TGT CGC TAT GAC | Transgene Reverse | A | |||
TmoIMR0076 | Fluorophore-1 | CAT TGG ACT CAT GGT GGG CGG TG | Quencher-1 | Tg Probe | ||
TmoIMR0105 | Fluorophore-2 | CCA ATG GTC GGG CAC TGC TCA A | Quencher-2 | IC Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
oIMR1544 | 0.40 uM |
oIMR3580 | 0.40 uM |
oIMR5260 | 0.40 uM |
oIMR7707 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
006409 | 129S1.129(Cg)-Tg(APPSw)40Btla/Mmjax |
031988 | 129S4.Cg-Tg(APPSwe,tauP301L)1Lfa Psen1tm1Mpm/LfaJ |
006555 | A.129(B6)-Tg(APPSw)40Btla/Mmjax |
005300 | B6.129-Tg(APPSw)40Btla/Mmjax |
005301 | B6.129S2-Tg(APP)8.9Btla/J |
006406 | B6.129S4-Tg(APPSwLon)96Btla/Mmjax |
004661 | B6.Cg-Zbtb20Tg(PDGFB-APPSwInd)20Lms/1J |
006293 | B6.Cg-Zbtb20Tg(PDGFB-APPSwInd)20Lms/2Mmjax |
005866 | B6.Cg-Tg(APP695)3Dbo Tg(PSEN1dE9)S9Dbo/Mmjax |
029770 | B6.Cg-Tg(APPswe,PSEN1dE9)85Dbo Apptm1Dbo/Mmjax |
008730 | B6.Cg-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax |
004662 | B6.Cg-Tg(PDGFB-APP)5Lms/J |
006006 | B6.Cg-Tg(Prnp-APP)A-2Dbo/J |
006005 | B6.Cg-Tg(Prnp-App/APPswe)E1-2Dbo/Mmjax |
007051 | B6.Cg-Tg(tetO-APPSwInd)102Dbo/Mmjax |
007052 | B6.Cg-Tg(tetO-APPSwInd)107Dbo/Mmjax |
007049 | B6.Cg-Tg(tetO-APPSwInd)885Dbo/Mmjax |
004807 | B6;129-Tg(APPSwe,tauP301L)1Lfa Psen1tm1Mpm/Mmjax |
004462 | B6;C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax |
006004 | B6C3-Tg(tetO-APPSwInd)885Dbo/Mmjax |
003375 | C3B6-Tg(APP695)3Dbo/Mmjax |
007027 | C57BL/6-Tg(Thy1-APPSwDutIowa)BWevn/Mmjax |
006472 | D2.129(B6)-Tg(APPSw)40Btla/Mmjax |
035561 | STOCK Tc(HSA21,CAG-EGFP)1Yakaz/J |
24 strains use this protocol |