B6.Cg-Tg(Prnp-App/APPswe)E1-2Dbo/Mmjax

Stock number: 006005
Research areas: Neurobiology Research

Description

These transgenic mice express a chimeric mouse/human amyloid precursor protein (APPswe) under the control of the mouse prion protein promoter. This mutant mouse strain may be useful in studies of Alzheimer's Disease.

Detailed description

These transgenic mice express a chimeric mouse/human amyloid precursor protein (APPswe) under the control of the mouse prion protein promoter. Mice that are hemizygous for the transgene are viable and fertile. More than half of the female hemizygous mice do not survive past 15 months of age. This mutant mouse strain may be useful in studies of Alzheimer's Disease.

Development

A transgenic construct a chimeric mouse/human amyloid precursor protein (APPswe) under the control of the mouse prion protein promoter was injected into fertilized C57BL/6J X C3HeJ F2 mouse eggs. Founder line E1-2 was established. The mice were then backcrossed to C57BL/6J for more than 14 generations.

Allele

Symbol: Tg(Prnp-App/APPswe)E1-2Dbo
Name: transgene insertion E1-2, David R Borchelt
MGI accession ID: MGI:3696473
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Prnp-App/APPswe)E1-2Dbo
Marker name: transgene insertion E1-2, David R Borchelt
Chromosome: UN
Strain of origin: (C57BL/6J x C3H/HeJ)F2
Expressed genes: APP,amyloid beta precursor protein,human
Molecular note: This transgene expresses a modified mouse amyloid precursor protein cDNA encoding the 695-amino acid isoform with a "humanized" Abeta domain amino acid sequence that includes the familial Alzheimer disease- (FAD-) associated Swedish double mutation (Lys595Asn and Met596Leu). The cDNA was inserted into an expression cassette containing the promoter, 5' intronic and 3' untranslated region sequences of the mouse prion protein gene.
General note: Three transgenic lines were generated and designated by the authors lines Q2-2, E1-2 and C3-3 (Tg(APP695)3Dbo). They were estimated, on the basis of Southern blot analysis, to carry 3-10 copies of the transgene.