These transgenic mice express the human amyloid precursor protein (APP) under the control of the mouse prion protein promoter. Mice that are hemizygous for the transgene are viable and fertile. This mutant mouse strain may be useful in studies of Alzheimer's Disease.

Development

A transgenic construct containing the human amyloid precursor protein (APP) under the control of the mouse prion protein promoter was injected into fertilized C57BL/6J mouse eggs. Founder line A-2 was established. The mice were then maintained by crossing hemizgyous male mice to C57BL/6J female mice.

Expression Data

Expressed Gene	APP, amyloid beta precursor protein, human
Site of Expression

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Genetics

Tg(Prnp-APP)A-2Dbo

Allele Symbol: Tg(Prnp-APP)A-2Dbo

Allele Name	transgene insertion A-2, David R Borchelt
Allele Type	Transgenic (Humanized sequence, Inserted expressed sequence)
Allele Synonym(s)
Gene Symbol and Name	Tg(Prnp-APP)A-2Dbo, transgene insertion A-2, David R Borchelt
Gene Synonym(s)
Promoter	Prnp, prion protein, mouse, laboratory
Expressed Gene	APP, amyloid beta precursor protein, human
Strain of Origin	C57BL/6
Chromosome	UN
Molecular Note	Mice with this transgene express the human amyloid precursor protein (APP) under control of the mouse prion protein promoter.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Alzheimer's Disease

Genotype: APP related

Neurobiology Research
- Alzheimer's Disease
- Neurodegeneration

Mammalian Phenotype Terms by Genotype

Genotype: Tg(Prnp-APP)A-2Dbo/0
B6.Cg-Tg(Prnp-APP)A-2Dbo/J

normal phenotype

no abnormal phenotype detected
- hemizygotes are viable and fertile; no further phenotypic analysis available

References

Additional - Tg(Prnp-APP)A-2Dbo related

Borchelt DR. 2007. Transgenic mice expressing human amyloid precursor protein under the control of the mouse prion protein promoter MGI Direct Data Submission :. MGI: J:117538

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Generic Tg(APP)
- MELT: Generic Tg(APP) Melt Curve Analysis
- Standard PCR: Tg(APP) cDNA
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice are bred as hemizygotes.
- Additional Breeding and Husbandry Support
Citation

When using the B6.Cg-Tg(Prnp-APP)A-2Dbo/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006006 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Not-For-Profit & Academic Pricing

Service	Genotype	Price
	Hemizygous or Non carrier for Tg(Prnp-APP)A-2Dbo

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Not Available to Companies or for Commercial Use

Strain(s) not available to companies or for-profit entities.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

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