| Allele Name | transgene insertion 20, Lennart Mucke |
| Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
| Allele Synonym(s) | APP Tg; APP/J20; hAPPJ20; hAPP-J20; J20; PDAPP-J20; PDGF-APPSwInd; PDGF-hAPP695,751,770V171F, KM670/671NL; Tg(PDGFB-APP*Swe*Ind)20Lms |
| Gene Symbol and Name | Zbtb20, zinc finger and BTB domain containing 20 |
| Gene Synonym(s) | |
| Promoter | PDGFB, platelet derived growth factor subunit B, human |
|---|
| Expressed Gene | APP, amyloid beta precursor protein, human |
|---|
| Site of Expression | APPSwInd is expressed in cerebral neurons, with the highest level in the neocortex and hippocampus. |
|---|
| Strain of Origin | (C57BL/6 x DBA/2)F2 |
| Chromosome | 16 |
| General Note | This line is also called line J20. Diffuse amyloid beta peptides deposition forms in the dendate gyrus and neocortex at 5 to 7 months of age. Amyloid deposition is progressive, with all transgenic mice exhibiting plaques by age 8 to 10 months. |
| Molecular Note | The transgene expresses the mutant human amyloid protein precursor APPSwInd, which bears both the Swedish (K670N/M671L) and the Indiana (V717F) mutations, under the control of the human platelet derived growth factor, B polypeptide (PDGFB) promoter. Hemizygous transgenic mice express immunodetectable transgene product in cerebral neurons, with the highest level of expression occurring in the neocortex and hippocampus. ELISA analysis reveals approximate total amyloid beta peptides and 42 amino acid length amyloid beta peptides in neocortical and hippocampal tissue from transgenic mice. Line 20 inserted into an intron of the gene at 43086322-43127049 (Build GRCm38/mm10) resulting in a 40,727 bp deletion. Founder line 20 has a copy number of greater than 10. |
| Mutations Made By | Lennart Mucke, Gladstone Inst of Neurological Disease |
