These APP YAC mice express a transgene containing human amyloid beta precursor protein (APP), and human APP transcripts and protein(s) are detected at levels similar to the endogenous mouse products in the brain and other tissues.
Bruce Lamb, Indiana University School of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
These transgenic mice express all mRNA and protein isoforms of the wild-type human amyloid beta (A4) precursor protein, APP. The transgene expression level is equivalent to the level of endogenous mouse amyloid beta (A4) precursor protein (in the homozygous state). The expression pattern of the various protein isoforms of human APP mimics endogenous mouse gene expression patterns. These mice serve as a model for dosage imbalance for APP that occurs in Down syndrome and also provide a unique model to examine the regulation of APP isoforms, APP processing and amyloid beta metabolism and regulation.
A 650 kb YAC transgene containing entire human APP gene (and approximately 350 kb flanking sequence) was transfected into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Founder animals were backcrossed to C57BL/6J for 11 generations.
Expressed Gene | APP, amyloid beta precursor protein, human |
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Site of Expression |
Allele Name | transgene insertion 8.9, Bruce Lamb |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | APPYAC; hABetaPP; hAPP-YAC; Py8.9; Tg(APP)C9.24Jgh |
Gene Symbol and Name | Tg(APP)8.9Btla, transgene insertion 8.9, Bruce Lamb |
Gene Synonym(s) | |
Promoter | APP, amyloid beta precursor protein, human |
Expressed Gene | APP, amyloid beta precursor protein, human |
Strain of Origin | 129S2/SvPas |
Chromosome | UN |
Molecular Note | A 650 kb YAC containing the entire human APP gene and 350 kb of flanking sequence was randomly integerated into ES cells. Southern blot analysis confirmed that construct rearrangement did not occur and that the animals carried a single copy of the intact transgene. Quantitative RT-PCR analysis of transgenic and wild-type mice revealed that the levels of transgenic human alternate transcripts corresponded with those of the endogenous alternative transcripts. The human APP promoter drives expression in the brain, heart, kidney and testes. |
Mutations Made By | Bruce Lamb, Indiana University School of Medicine |
The strain is maintained as a homozygote.
When using the B6.129S2-Tg(APP)8.9Btla/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005301 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(APP)8.9Btla |
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