CD34+ Humanized NSG-FLT3 Mice

Appearance & Care

CD34+ hu-NSG-FLT3 Mouse - The Jackson Laboratory

While CD34+ hematopoietic stem cell humanized mice do have a human-like immune system on-board, it is not fully functional. These mice should still be handled very carefully and treated as an immuno-deficient strain. We are glad to offer the following resources to help you care for highly immuno-deficient strains like NSG™. Additionally, you should be sure to check with your institution regarding requirements for housing mice with engrafted human tissue.

Animal Health Reports

RB11 (Maximum)

Phenotype Information

CD34 humanized NSG-FLT3 mice demonstrate presence of CD45 cells following engraftment, compared to NSG and NSG-SGM3 strains
CD34 humanized NSG-FLT3 mice demonstrate elevated levels of CD33+ myeloid progenitors after engraftment, compared to NSG and NSG-SGM3 strains
CD34 humanized NSG-FLT3 mice demonstrate elevated levels of CD14+ monocytes after engraftment, compared to NSG and NSG-SGM3 strains

Figure 1. CD34 humanized NSG-FLT3 mice demonstrate presence of CD45 cells (left) and elevated levels of CD33+ myeloid progenitors (center) and CD14+ monocytes (right) following engraftment as compared to NSG and NSG-SGM3 strains.

CD34 humanized NSG-FLT3 mice show elevated levels of CD3+ T cells following engraftment as compared to the NSG strain
CD34 humanized NSG-FLT3 mice show elevated levels of immature natural killer cells after engraftment, compared to the NSG strain

Figure 2. CD34 humanized NSG-FLT3 mice elevated levels of CD3+ T cells (left) and immature natural killer cells (right) following engraftment as compared to the NSG strain.

CD34 humanized NSG-FLT3 demonstrate increased presence of conventional dendritic cells following engraftment

Figure 3. CD34 humanized NSG-FLT3 demonstrate increased presence of conventional dendritic cells following engraftment.

NSG-FLT3 mice demonstrate TNF cytokine release after innervation of TLR4 LPS receptors following immuno-stimulation
NSG-FLT3 mice demonstrate TNF cytokine release after innervation of TLR3 Poly I:C receptors following immuno-stimulation
NSG-FLT3 mice demonstrate TNF cytokine release after innervation of TLR9 CpG receptors following immuno-stimulation
NSG-FLT3 mice demonstrate IL12p40 cytokine release after innervation of TLR4 LPS receptors following immuno-stimulation
NSG-FLT3 mice demonstrate IL12p40 cytokine release after innervation of TLR3 Poly I:C receptors following immuno-stimulation
NSG-FLT3 mice demonstrate IL12p40 cytokine release after innervation of TLR9 CpG receptors following immuno-stimulation

Figure 4. NSG-FLT3 mice demonstrate TNF and IL12p40 cytokine release following innervation of TLR4 (LPS, left column), TLR3 (Poly I:C, center column), and TLR9 (CpG, right column) receptors following immuno-stimulation.

CD34 humanized NSG-FLT3 successfully engraft patient-derived PS4050 melanoma
CD34 humanized NSG-FLT3 successfully engraft patient-derived PS4050 melanoma and demonstrate infiltration of T cells in the tumor
CD34 humanized NSG-FLT3 successfully engraft patient-derived PS4050 melanoma and demonstrate infiltration of conventional dendritic cells in the tumor
CD34 humanized NSG-FLT3 successfully engraft patient-derived PS4050 melanoma and demonstrate infiltration of plasmacytoid dendritic cells in the tumor
CD34 humanized NSG-FLT3 successfully engraft patient-derived PS4050 melanoma and demonstrate infiltration of monocytes/macrophages in the tumor
CD34 humanized NSG-FLT3 successfully engraft patient-derived PS4050 melanoma and demonstrate infiltration of polarized M1 cells in the tumor
CD34 humanized NSG-FLT3 successfully engraft patient-derived PS4050 melanoma and demonstrate infiltration of polarized M2 cells in the tumor

Figure 5. CD34 humanized NSG-FLT3 successfully engraft patient-derived PS4050 melanoma and demonstrate infiltration of T cells, conventional dendritic cells, plasmacytoid dendritic cells, monocytes/macrophages, and polarized M1/M2 cells in the implanted tumor.

References & Resources

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