FVB.129P2(B6)-Trp53^tm1Brn/JwkimJ

Stock number: 034624
Product name: FVB.p53^LoxP
Strain synonyms: FVB.p53 flox

Research areas: Cancer Research, Research Tools

Description

When these p53^flox mice are bred to mice with a Cre recombinase gene under the control of a promoter of interest, Trp53 expression is deleted in the tissue of interest.

Of note, The Jackson Laboratory Repository also offers p53^flox mice on a C57BL/6 genetic background (Stock No. 008462).

Detailed description

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. The phenotype summarized below is for the parental line: p53^flox mice on a C57BL/6 genetic background (Stock No. 008462). It should be noted that the phenotype of this FVB/NJ-congenic p53^flox line (Stock No. 034624) could vary from that of the parental line from which it was derived.

Exons 2-10 are flanked by loxP sites in this conditional targeted mutant strain. Mice homozygous for the floxed allele do not show any increase in disease incidence for at least a year. When bred to mice with a cre recombinase gene under the control of a promoter of interest, expression is deleted in the tissue of interest.

For example, when crossed to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771), this mutant mouse strain may be useful in studies of medulloblastoma formation.

When crossed to a strain expressing Cre recombinase in the central nervous system (see Stock No. 004600), this mutant mouse strain may be useful in studies of astrocytoma formation.

When crossed to a strain expressing Cre recombinase in virgin and lactating mammary glands (see Stock No. 003553), this mutant mouse strain may be useful in studies of mammary gland tumors.

When crossed to a strain expressing a doxycyclin-inducible Cre recombinase in the osteoblast lineage (see Stock No. 006361), this mutant mouse strain may be useful in studies of osteosarcomas.

When crossed to a strain expressing Cre recombinase in the epithelial cells of the developing kidney and genitourinary tract (see Stock No. 012237), this mutant mouse strain may be useful in studies of endometrial carcinoma.

Development

A targeting vector was used to introduce flanking loxP sites to introns 1 and 10 of the gene. An IB10/E14IB10 129P2/OlaHsd-derived embryonic stem cell line was used to create the mutation. This line was backcrossed to C57BL/6 for eight generations before being donated to The Jackson Laboratory in 2008 as Stock No. 008462.

In 2013, Dr. Jung-Whan Kim purchased some Stock No. 008462 animals (~N10 onto C57BL/6) and then backcrossed them ten generations to FVB/NJ inbred mice (from Stock No. 001800). The resulting FVB/NJ-congenic strain is called FVB.p53^flox. Of note, the donating investigator reports that, at least once during backcrossing, a p53^flox female was bred to an FVB/NJ inbred male (thus the Y chromosome of the congenic strain is of FVB/NJ origin). In 2020, FVB.p53^flox males (white coat color) were sent to The Jackson Laboratory Repository as Stock No. 034624. Upon arrival, males were used to cryopreserve sperm. To establish our live colony, an aliquot of frozen sperm was used to fertilize FVB/NJ oocytes (Stock No. 001800).

Allele

Symbol: Trp53^tm1Brn
Name: targeted mutation 1, Anton Berns
MGI accession ID: MGI:1931011
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: p53^Co; p53^f; p53^F2-10; p53^F2-10F; p53^fl; p53^flox; p53^L; p53^lox; p53^LoxP; p53Fl; Tp53^flox; Tp53^flx; Trp53^f; Trp53^F2-10; Trp53^F2-F10; Trp53^Fl; Trp53^lox; Trp53^loxP; Trp53-loxP flanked; Trp53^Flox
Marker symbol: Trp53
Marker name: transformation related protein 53
Marker synonyms: BCC7; BMFS5; LFS1; p44; p53; TRP53
Chromosome: 11
Strain of origin: 129P2/OlaHsd
Molecular note: Insertion of loxP sites flanking exons 2 through 10. No effect on the normal function of the gene.