This C3H/HeJ congenic carrying a null allele for the Prdm9 histone methytransferase provides a model for investigations into meiotic recombination and genetically defined recombination hotspots.
Mary Ann Handel, The Jackson Laboratory
This C3H/HeJ congenic carrying a null allele for the Prdm9 histone methytransferase provides a model for investigations into meiotic recombination and genetically defined recombination hotspots. On this C3H/HeJ genetic background, homozyogous males and females exhibit infertility due to prophase I meiotic arrest, similar to what is found on the C57BL/6J genetic background. However, females homozygous for this null allele on the CAST/EiJ genetic background have many more oocytes that survive and do not show decreased fertility. Powers et al. traced that sexually dimorphic phenotype to a dominant CAST modifier on Chromosome 5. (Powers et al., 2020.)
The Prdm9tm1Ymat null allele was backcrossed from the B6;129P2-Prdm9tm1Ymat/J segregating genetic background onto C3H/HeJ for 10 backcrossing generations in the laboratory of Dr. Mary Ann Handel. Sperm was cryopreserved from heterozygous males.
|Allele Name||targeted mutation 1, Yasuhisa Matsui|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Prdm9, PR domain containing 9|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A targeting vector containing the lacZ gene and a loxP-flanked PGKneo cassette disrupted part of exon 2, which contains the initiation codon, all of exons 3 to 6, and part of exon 7. RT-PCR analysis of testes from homozygous mutant mice did not detect transcript.|
|Mutations Made By|| |
Yasuhisa Matsui, Tohoku University
Due to infertility in both homozygous females and males this strain must be maintained using a heterozygous breeder and homozygotes can only be generated from a heterozgyous pair.
When using the C3.129P2(B6)-Prdm9tm1Ymat/HandJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #033547 in your Materials and Methods section.