Overview

Also Known As:B6J hAbeta

This B6J hAbeta mutant strain carries a CRISPR/Cas9-generated App allele with a humanized Abeta1-42 region (G601R, F606Y, R609H in the mouse gene, corresponding to amino acid positions 676, 681, 684 in the human APP locus). These mice may be suitable for use in studies related to Alzheimer's disease.

Donating Investigator

Mike Sasner, The Jackson Laboratory

Genetic overview

Genetic Background	Generation

App^em1Adiuj

Allele Type	Gene Symbol	Gene Name
Endonuclease-mediated (Humanized sequence)	App	amyloid beta (A4) precursor protein

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Research Applications

Neurobiology Research
Research Tools

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Details

Detailed Description

This hAbeta mutant strain carries a mutant allele of the App gene containing G601R, F606Y, and R609H point mutations. The targeted App gene encodes amyloid beta precursor protein, a transmembrane cell surface receptor that is cleaved by secretases. Mutations in this gene have been associated with Alzheimer's disease.

Both heterozygous and homozygous mice are viable and fertile (March 2020). If additional characterization of these hAbeta mice is performed, we may modify the strain description accordingly.

Development

This mutant line was generated by breeding the Apoe^{tm1.1(APOE*4)Adiuj} and Trem2^em1Adiuj/J alleles out of Stock No. 030670.

Important note about App isoforms and exon numbering: The App-201 isoform (695 aa protein) is encoded by 16 exons, of which the Abeta sequence is encoded by exon 14. The App-206 isoform (770 aa protein) is encoded by 18 exons, of which the Abeta sequence is encoded by exon 16.

Control Suggestions

Wild-type from the colony

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Genetics

App^em1Adiuj

Allele Symbol: App^em1Adiuj

Allele Name	endonuclease-mediated mutation 1, MODEL-AD Center
Allele Type	Endonuclease-mediated (Humanized sequence)
Allele Synonym(s)
Gene Symbol and Name	App, amyloid beta (A4) precursor protein
Gene Synonym(s)
Strain of Origin	B6(SJL)-Apoe^{tm1.1(APOE*4)Adiuj} Trem2^em1Adiuj/J
Chromosome	16
Molecular Note	CRISPR/Cas9 genome editing methodologies are used to introduce G601R, F606Y, and R609H point mutations into the App gene. G601R corresponds to a GGA to CGA nucleotide change, F606Y represents a TTT to TAT change, and R609H incorporates a CGC to CAT nucleotide change.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Alzheimer's Disease
  - strains expressing mutant APP
  - APP mutants
Research Tools
- Neurobiology Research

Mammalian Phenotype Terms by Genotype

References

Additional - App^em1Adiuj related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:App
- Genotyping resources and troubleshooting
Breeding Considerations

Both heterozygous and homozygous mice are viable and fertile.
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
Alternatively, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Citation
When using the B6J hAbeta mouse strain in a publication, please cite the originating article(s) and include JAX stock #033013 in your Materials and Methods section.

Strain Interest Registration

Please fill out the form below to indicate your interest in purchasing this JAX®Mice strain.
This information helps us manage the colony build and better meet the broad needs of the research community.
Please send any technical questions to Technical Support.

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Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

The use of this mouse model is subject to the terms and conditions of the Limited License from The Broad Institute.

The use of this mouse model is subject to the terms and conditions of the Limited Use Label License from Caribou Biosciences, Inc.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:B6J hAbeta

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Appem1Adiuj

Allele Symbol: Appem1Adiuj

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Appem1Adiuj related

Genotyping Protocols

Breeding Considerations

Citation

Strain Interest Registration

Contact Information

Interest

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

App^em1Adiuj

Allele Symbol: App^em1Adiuj

Additional - App^em1Adiuj related