This CRISPR-generated knock-out mutant of the arginine vasopressin receptor 1A (Avpr1a) gene has been generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory. Avpr1a encodes a G-protein-coupled receptor that mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis.
The Jackson Laboratory
This strain was generated by the Knockout Mouse Phenotyping Program (KOMP3) at The Jackson Laboratory using CRISPR technology. The targeted gene, arginine vasopressin receptor 1A (Avpr1a), encodes a G-protein-coupled receptor that mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis. The alteration resulted in the deletion of 1550 bp in exon 1 and 259 bp of flanking intronic sequence including the start of translation and splice donor and is predicted to result in a null allele. As mice are characterized, phenotype data will be accessible on the International Mouse Phenotyping Consortium website.
Guide RNAs (CAACAAACTCAGCCCATTAG and GAAGCTACCTGCAATCTGGA) were designed to insert create a 1550 bp deletion in exon 1 of the arginine vasopressin receptor 1A (Avpr1a) gene beginning at Chromosome 10 position 122,448,382 bp and ending after 122,449,931 bp (GRCm38/mm10). The mutation is predicted to delete ENSMUSE00000100823 (exon 1) and 259 bp of flanking intronic sequence including the start of translation and splice donor and is predicted to result in a null allele. Guide RNAs and Cas9 nuclease were introduced into C57BL/6NJ-derived fertilized eggs with well recognized pronuclei. Embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by sequencing and further bred to C57BL/6NJ (Stock No. 005304) to develop the colony.
|Allele Name||endonuclease-mediated mutation 1, Jackson|
|Allele Type||Endonuclease-mediated (Null/Knockout)|
|Gene Symbol and Name||Avpr1a, arginine vasopressin receptor 1A|
|Strain of Origin||C57BL/6NJ|
|Molecular Note||This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences AGGAGGCTGGAGAAATTCCG and TAGAAGCTTAACTATTGAAT, which resulted in a 1550 bp deletion beginning at Chromosome 10 position 122,448,382 bp and ending after 122,449,931 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000100823 (exon 1) and 259 bp of flanking intronic sequence including the start of translation and splice donor and is predicted to result in a null allele.|
Heterozygotes may be bred to C57BL/6NJ (Stock No. 005304) or wildtype littermates. As mice are characterized, data related to viability and fertility will be provided on the International Mouse Phenotyping Consortium website.
When using the C57BL/6NJ-Avpr1aem1(IMPC)J/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #46134 in your Materials and Methods section.
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