These MMTV-NeuNDL2-5-IRES-Cre transgenic mice express an activated Erbb2 (oncogenic form NDL2-5, neu deletion, neu8342) and cre recombinase under control of the mammalian mammary tumor virus (MMTV) promoter, and develop mammary tumors with a mean latency of 146 days.
Dr. William J. Muller, McGill University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Inserted expressed sequence) |
These MMTV/NIC transgenic mice express activated, oncogenic human ERBB2 (neu8342, NDL2-5, neu deletion) and cre recombinase under control of the mammalian mammary tumor virus (MMTV) promoter. Transgene expression is detected in mammary gland, with lower expression detected in adrenal gland, lung, ovary, pancreas and salivary gland. Hemizygous mice develop mammary tumors with a mean latency of 146 days. Tumors develop only in mammary gland. The tumors are histologically characterized as solid/nodular adenocarcinomas, molecularly resembling human luminal breast cancer. During backcrossing, the Y chromosome may not have been fixed to the FVB genetic background Hemizygotes are viable and fertile. Homozygous viability/fertility has not been tested (February 2019).
A transgenic construct containing an activated, oncogenic human ERBB2 (neu8342, NDL2-5, neu deletion) cDNA and IRES-cre, under the control of the MMTV (mammalian mammary tumor virus) promoter, was injected into fertilized FVB/N mouse eggs. Founder line 1 was subsequently established. The mice were then backcrossed to FVB for 15 generations. During backcrossing, the Y chromosome may not have been fixed to the FVB genetic background. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize FVB/NJ oocytes (Stock No. 001800).
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Expressed Gene | Erbb2, erb-b2 receptor tyrosine kinase 2, mouse, laboratory |
Site of Expression |
Allele Name | transgene insertion 1, William J Muller |
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Allele Type | Transgenic (Recombinase-expressing, Inserted expressed sequence) |
Allele Synonym(s) | MMTV-Neu-IRES-CRE; MMTV-NeuNDL2-5-IRES-Cre; MMTV-NIC; NIC |
Gene Symbol and Name | Tg(MMTV-Erbb2*,-cre)1Mul, transgene insertion 1, William J Muller |
Gene Synonym(s) | |
Promoter | MMTV, Mouse Mammary Tumor Virus, MMTV |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Expressed Gene | Erbb2, erb-b2 receptor tyrosine kinase 2, mouse, laboratory |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | A transgene was generated where an activated Erbb2 (Neu, oncogenic form NDL2-5) and cre recombinase (coupled by an internal ribosomal entry site (IRES)) are under control of the mammalian mammary tumor virus promoter (MMTV). |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to FVB/NJ inbred mice (Stock No. 001800). The Donating Investigator has not attempted to make the strain homozygous. Homozygous viability/fertility has not been tested (February 2019).
When using the MMTV-Neu-IRES-CRE, MMTV-NeuNDL2-5-IRES-Cre, MMTV-NIC, NIC mouse strain in a publication, please cite the originating article(s) and include JAX stock #032576 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non-carrier for Tg(MMTV-Erbb2*,-cre)1Mul |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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