Il17rcfl/fl mice possess loxP sites flanking exons 2-3 of Il17rc making them useful for tissue specific IL-17rc deficiency. These floxed mice are useful when studying inflammatory and autoimmune diseases.
Jay Kolls, University of Pittsburgh School of Medicine
Il17rcfl/fl mice possess loxP sites flanking exons 2-3 of the interleukin 17 receptor C (Il17rc) gene. IL-17RC is a protein with high affinity for the receptor of proinflammatory cytokine IL-17. IL-17RC is expressed in nonhemopoietic tissues, and binds both IL-17A and IL-17F which are involved in the progression of inflammatory and autoimmune diseases. It can also form a heterodimer with IL-17RC, which together plays a critical role in host defense against extracellular bacterial and fungal pathogens. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 2-3 deleted in the cre-expressing tissues.
When bred to mice expressing Cre Recombinase in epithelial cells of the small and large intestines, resulting IL-17RC deficient mice lack IL-17A and IL-17F signaling and a reduction in H2O2 concentrations in the terminal ileum.
The Il17rcfl targeting vector was designed to insert a loxP site upstream of exon 2 and a frt-flanked neomycin resistance (neo) cassette, followed by a second loxP site, was inserted downstream of exon 3 of the interleukin 17 receptor C (Il17rc) gene. The construct was electroporated into B6(Cg)-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with C57BL/6J mice (Stock No. 000664). Offspring were bred to Flp Recombinase expressing strain at OzGene to delete the neo cassette. Progeny were bred with C57BL/6J mice to remove the Flp-expressing transgene. Resulting Il17rcfl/fl mice were bred with C57BL/6J mice for at least 5 generations. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes.
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers, on Chromosome 3, was segregating. Also, 2 of the 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6N;C57BL/6J genetic background.
|Allele Name||targeted mutation 1.1, Jay K Kolls|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Il17rc, interleukin 17 receptor C|
|Strain of Origin||B6.Cg-Thy1a|
|Molecular Note||A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted upstream of exon 4. Flp-mediated recombination removed the FRT-flanked neo cassette.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Il17rcfl mouse strain in a publication, please cite the originating article(s) and include JAX stock #031002 in your Materials and Methods section.
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