Il17rcfl/fl mice possess loxP sites flanking exons 2-3 of Il17rc making them useful for tissue specific IL-17rc deficiency. These floxed mice are useful when studying inflammatory and autoimmune diseases.
Jay Kolls, University of Pittsburgh School of Medicine
Il17rcfl/fl mice possess loxP sites flanking exons 2-3 of the interleukin 17 receptor C (Il17rc) gene. IL-17RC is a protein with high affinity for the receptor of proinflammatory cytokine IL-17. IL-17RC is expressed in nonhemopoietic tissues, and binds both IL-17A and IL-17F which are involved in the progression of inflammatory and autoimmune diseases. It can also form a heterodimer with IL-17RC, which together plays a critical role in host defense against extracellular bacterial and fungal pathogens. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 2-3 deleted in the cre-expressing tissues.
When bred to mice expressing Cre Recombinase in epithelial cells of the small and large intestines, resulting IL-17RC deficient mice lack IL-17A and IL-17F signaling and a reduction in H2O2 concentrations in the terminal ileum.
The Il17rcfl targeting vector was designed to insert a loxP site upstream of exon 2 and a frt-flanked neomycin resistance (neo) cassette, followed by a second loxP site, was inserted downstream of exon 3 of the interleukin 17 receptor C (Il17rc) gene. The construct was electroporated into B6(Cg)-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with C57BL/6J mice (Stock No. 000664). Offspring were bred to Flp Recombinase expressing strain at OzGene to delete the neo cassette. Progeny were bred with C57BL/6J mice to remove the Flp-expressing transgene. Resulting Il17rcfl/fl mice were bred with C57BL/6J mice for at least 5 generations. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes.
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers, on Chromosome 3, was segregating. Also, 2 of the 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6N;C57BL/6J genetic background.
|Allele Name||targeted mutation 1.1, Jay K Kolls|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||targeted mutation 1.1, Jay K Kolls; Il17rctm1.1Koll|
|Gene Symbol and Name||Il17rc, interleukin 17 receptor C|
|Gene Synonym(s)||1110025H02Rik; IL17-RL; IL17RL; Il17rl; interleukin 17 receptor-like; 1110025H02Rik; RIKEN cDNA 1110025H02 gene; CANDF9; Il17rl|
|Strain of Origin||B6.Cg-Thy1a|
|Molecular Note||A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted upstream of exon 4. Flp-mediated recombination removed the FRT-flanked neo cassette.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Il17rcfl mouse strain in a publication, please cite the originating article(s) and include JAX stock #031002 in your Materials and Methods section.
|Heterozygous for Il17rc<tm1.1Koll>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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