Prdx6 KO mice exhibit an increased sensitivity to oxidative stress. This strain may be useful for studying the role of peroxidase phospholipids in cell membranes and the recovery of multiple cells types from peroxidative stress.
Aron B. Fisher, University of Pennsylvania
PRDX6 (peroxiredoxin 6) is a bifunctional enzyme with phospholipase A2 and glutathione peroxidase activity and is involved in protection from oxidative stress. Lungs from mice homozygous for the Prdx6 knockout (KO) allele exhibit an increased sensitivity to lung injury following exposure to oxygen at elevated partial pressure or administration of paraquat. Similarly, lungs or pulmonary microvascular endothelial cells exposed to hypoxia exhibit a markedly delayed recovery.
KO mice subjected to hepatic ischemia-reperfusion (I/R) injury develop greater hepatocellular injury and increased mitochondrial generation of H2O2 and dysfunction. In the heart, I/R in KO mice results in reduced recovery of left ventricular function, increased myocardial infarct size and increased numbers of apoptotic cardiomyocytes.
Mice homozygous for the mutation are viable and fertile. This strain may be useful for studying the role of peroxidase phospholipids in cell membranes and the recovery of multiple cells types from peroxidative stress.
A targeting vector containing a neomycin resistance (neo) cassette, in reverse orientation to the gene, was used to disrupt part of intron 2 and exon 3. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6J mice for at least 7 generations to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice for at least one generation.
|Allele Name||targeted mutation 1, Aron B Fisher|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||1-cysPrx -|
|Gene Symbol and Name||Prdx6, peroxiredoxin 6|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Part of intron 2 and the first part of exon 3 were replaced by a neomycin resistance gene in the reverse orientation. Lack of gene product in homozygotes was demonstrated by both real-time PCR and Western blot analysis.|
When maintaining a live colony, these mice can be maintained as homozygotes.
When using the 1-cysPrx KO mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #43402 in your Materials and Methods section.