Overview

Also Known As: C3(1)/Tag-REAR

C3(1)/TAg-REAR mice allow transplantation of TAg-expressing tumors and/or cell lines in the FVB/N genetic background, and are a novel immunocompetent transplant model for studying basal-like triple negative breast cancer, prostate tumors from TRAMP mice, C3(1)/TAg tumor cell lines, etc. In contrast to the higher-transgene copy C3(1)/TAg line (Stock No. 013591) from which it was derived, C3(1)/TAg-REAR mice have only one copy (or a partial copy) of the transgene - resulting in retained tolerance to SV40 TAg but no spontaneous cancer phenotype.

Donating Investigator

Jeffrey E Green, National Cancer Institute, National Institutes of Health (NIH/NCI)

Olga Aprelikova, National Cancer Institute, National Institutes of Health (NIH/NCI)

Genetic overview

Genetic Background	Generation
	?+pN1 (2017-12-11 00:00:00)

Tg(C3-1-TAg)cJeg

Allele Type
Transgenic (Inserted expressed sequence)

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Research Applications

Reproductive Biology Research
Research Tools
Cancer Research

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Base Price

Starting at:

$255.00 Domestic price for female 4-week

510.00 Domestic price for breeder pair

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Details

Detailed Description

The C3(1)/TAg transgene has the 5' flanking region of the rat prostatic steroid binding protein gene [C3(1)] directing expression of the early region of simian virus 40 large tumor antigen (TAg) to prostate and mammary gland tissues. The original C3(1)/TAg founder line C contained ~six transgene copies at a single locus in the telomeric portion of chromosome 6 (which contains the Ki-ras proto-oncogene) - resulting in multistage oncogenesis in prostate and mammary gland (see Stock No. 013591).

From that original line, the C3(1)/TAg-REAR subline (Stock No. 030386) was identified with transgene rearrangement/deletion to only one copy (or a partial copy) in the original chromosome 6 locus.
As a result, C3(1)/TAg-REAR mice exhibit no spontaneous cancer phenotype and retain immunological tolerance to cells expressing TAg (including tumors from the C3(1)/TAg and TRAMP models).
C3(1)/TAg-REAR have a normally functioning immune system - as evidenced by a normal complement of subclasses of immune cells and the rejection of both human tumor cells and murine tumors derived from a non-FVB/N background.
Additionally, induction of an anti-tumor immune response can be elicited in C3(1)/TAg-REAR mice (e.g., irradiated C3(1)/TAg tumor cell vaccination significantly inhibits growth of injected C3(1)/TAg-derived mammary fat pad tumors).
Furthermore, C3(1)/TAg tumors or cell lines can be implanted synchronously into the mammary fat pads of large cohorts of immunocompetent C3(1)/TAg-REAR mice - this bypasses the need to wait for tumors to develop spontaneously in multiple transgenic mice over a variable time course and allows therapeutic interventions to be performed in a relatively short timeframe.

Mice hemizygous or homozygous for the C3(1)/TAg-REAR transgene are viable with no spontaneous overt phenotype. Hemizygotes are fertile with no breeding problems. The fertility of homozygous females has not been characterized to date (May 2017). The donating investigator reports homozygous males are fertile when bred to FVB/N females (July 2018).

Development

The C3(1)/TAg transgene was designed by Dr. Jeffrey E. Green (NIH/NCI) to have the 5prime flanking region of the rat prostatic steroid binding protein gene [C3(1)] followed by the sequences encoding the early region of simian virus 40 large tumor antigen (TAg).
The C3(1)/TAg transgene was microinjected into FVB/N fertilized oocytes. Founder line C was established with ~six copies of the transgene integrated at a single locus in the telomeric portion of chromosome 6 (which contains the Ki-ras proto-oncogene). C3(1)/TAg founder line C was maintained on the FVB/N genetic background (see Stock No. 013591). Routine screening of Dr. Green’s C3(1)/TAg founder line C colony identified female transgenic mice that retained tolerance to SV40 TAg but did not develop the expected phenotype (mammary tumors). These females were bred to FVB/NCrl wildtype males to propagate the new subline, called C3(1)/TAg-REAR. Characterization of C3(1)/TAg-REAR revealed a transgene rearrangement had occurred that deleted most of the original transgene copies - apparently leaving only one copy (or a partial copy) of the transgene in the original chromosome 6 locus. The C3(1)/TAg-REAR subline was then maintained by breeding hemizygous C3(1)/Tag-REAR males with FVB/NCrl females.
In 2017, Dr. Green sent albino males hemizygous for the C3(1)/TAg-REAR transgene on the FVB/N genetic background to The Jackson Laboratory as Stock No. 030386. Upon arrival, sperm was cryopreserved. To establish our living colony, an aliquot of the frozen sperm was used to fertilize FVB/NJ oocytes (Stock No. 001800).

Expression Data

Expressed Gene	TAg, SV40 large T-antigen, SV40
Site of Expression

Control Suggestions

Noncarrier

Approximate Controls

001800 FVB/NJ

Additional Information

Considerations for Choosing Controls

Selected References

Aprelikova O; Tomlinson CC; Hoenerhoff M; Hixon JA; Durum SK; Qiu TH; He S; Burkett S; Liu ZY; Swanson SM; Green JE. 2016. Development and Preclinical Application of an Immunocompetent Transplant Model of Basal Breast Cancer with Lung, Liver and Brain Metastases. PLoS One 11(5):e0155262PubMed: 27171183 MGI: J:240994
Maroulakou IG; Anver M; Garrett L; Green JE. 1994. Prostate and mammary adenocarcinoma in transgenic mice carrying a rat C3(1) simian virus 40 large tumor antigen fusion gene. Proc Natl Acad Sci U S A 91(23):11236-40PubMed: 7972041 MGI: J:52786

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Genetics

Tg(C3-1-TAg)cJeg

Allele Symbol: Tg(C3-1-TAg)cJeg

Allele Name	transgene insertion c, Jeffrey E Green
Allele Type	Transgenic (Inserted expressed sequence)
Allele Synonym(s)	Tg(C3-1-TAg)cJeg; transgene insertion c, Jeffrey E Green
Gene Symbol and Name	Tg(C3-1-TAg)cJeg, transgene insertion c, Jeffrey E Green
Gene Synonym(s)	C3(1)-SV40 T antigen; C3(1)/SV40T; C3(1)/T(AG); C3(1)/TAg; C3(1)/SV40; C3(1)/T antigen (Tag); Large T
Promoter	C3(1), C3(1), rat
Expressed Gene	TAg, SV40 large T-antigen, SV40
Strain of Origin	FVB/N
Chromosome	UN
Molecular Note	This transgene contains the rat prostatic binding protein C3 promoter (Pbpc3) and the wild-type allele of the SV40 large tumor antigen (TAg) gene. Founder c carried 6 copies of the transgene. Founders f, g, i, j, k, and l were also generated. Two transgenic lines (c and l) were produced.
Mutations Made By	Jeffrey Green, National Cancer Institute, National Institutes of Health (NIH/NCI)

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Reproductive Biology Research
- Prostate Tumors
- Gonadal Tumors
  - control strain
Research Tools
- Cancer Research
  - tumor immunology
  - xenograft/transplant host
- Toxicology Research
  - xenograft/transplant host
Cancer Research
- Toxicology
  - xenograft/transplant host
- Other
  - tumor metastasis

Mammalian Phenotype Terms by Genotype

References

Aprelikova O; Tomlinson CC; Hoenerhoff M; Hixon JA; Durum SK; Qiu TH; He S; Burkett S; Liu ZY; Swanson SM; Green JE. 2016. Development and Preclinical Application of an Immunocompetent Transplant Model of Basal Breast Cancer with Lung, Liver and Brain Metastases. PLoS One 11(5):e0155262PubMed: 27171183 MGI: J:240994
Maroulakou IG; Anver M; Garrett L; Green JE. 1994. Prostate and mammary adenocarcinoma in transgenic mice carrying a rat C3(1) simian virus 40 large tumor antigen fusion gene. Proc Natl Acad Sci U S A 91(23):11236-40PubMed: 7972041 MGI: J:52786

Additional - Tg(C3-1-TAg)cJeg related

Technical Support

Contact Technical Support

Genotyping Protocols
- High Resolution Melting: Tg(C3-1)cJeg
- QPCR: Generic SV40 TAg 1
- Genotyping resources and troubleshooting
Breeding Considerations

Mice hemizygous or homozygous for the C3(1)/TAg-REAR transgene are viable with no spontaneous overt phenotype. Hemizygotes are fertile with no breeding problems. The fertility of homozygous females has not been characterized to date (May 2017). The donating investigator reports homozygous males are fertile when bred to FVB/N females (July 2018).

When maintaining a live colony, hemizygous mice may be bred together, to wildtype mice from the colony or to FVB/NJ inbred mice (Stock No. 001800).
- Additional Breeding and Husbandry Support
Mating System
- Hemizygote x Hemizygote
Citation
When using the C3(1)/Tag-REAR mouse strain in a publication, please cite the originating article(s) and include JAX stock #030386 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX18 (Maximum)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service	Genotype	Price
	Hemizygous or non-carrier for Tg(C3-1-TAg)cJeg

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

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Also Known As: C3(1)/Tag-REAR

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Tg(C3-1-TAg)cJeg

Allele Symbol: Tg(C3-1-TAg)cJeg

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Tg(C3-1-TAg)cJeg related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability