FVB.Cg-Kdm2b^tm1.1Bes/J

Stock number: 029345
Product name: Fbxl10-2^flox
Research areas: Cell Biology Research, Research Tools

Description

Fbxl10-2^flox mice possess loxP sites flanking exon 1 of the Kdm2b isoform 2 gene. This strain may be useful for studying the role of DNA methylation during embryonic development.

Detailed description

Fbxl10-2^flox mice possess loxP sites flanking exon 1 of the lysine (K)-specific demethylase 2B (Kdm2b) isoform 2 gene (also termed Fbxl10-2). This region corresponds to intron 12 in the Kdm2b isoform 1 gene (Fbxl10-1). FBXL10-2 is a F-box protein occupying every unmethylated CpG-dense promoters; FBXL10-2 is composed by a CXXC motif, a PHD motif, a leucine rich region and a F-Box domain. FBXL10 has also been reported to be an oncogene and a tumor suppressor in leukemia. Complete inactivation of the Fbxl10 gene leads to methylation only of promoters that are co-occupied by both FBXL10 and Polycomb repressive complexes (PRCs). Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 1 of Fbxl10-2 deleted in the cre-expressing tissues. Cre-recombination does not affect Fbxl10-1 gene expression.

Development

A targeting vector was designed to insert loxP sites flanking exon 1 of the lysine (K)-specific demethylase 2B (Kdm2b) isoform 2 gene. A frt-flanked neomycin resistance (neo) cassette was inserted downstream of the second loxP site. This region corresponds to intron 12 in the Kdm2b isoform 1 gene. The construct was electroporated into C57bl6/129sv hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred with ROSA26:FLPe mice on a C57BL/6 background to delete the neo cassette. Offspring were bred to FVB/NJ mice for at least 8 generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize FVB/NJ oocytes (Stock No. 001800).

Allele

Symbol: Kdm2b^tm1.1Bes
Name: targeted mutation 1.1, Timothy H Bestor
MGI accession ID: MGI:5784548
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Marker symbol: Kdm2b
Marker name: lysine (K)-specific demethylase 2B
Chromosome: 5
Strain of origin: C57BL/6 x 129/Sv
Molecular note: The targeting vector is designed to insert a loxP site upstream of exon 1 (including the alternative internal promoter) followed by a second loxP site and an FRT-flanked neomycin resistance (neo) cassette downstream of exon 1 of isoform 2. This region corresponds to intron 12 in the Kdm2b isoform 1 gene. Flp-mediated recombination removed the FRT-flanked neo cassette.