Fbxl10-2flox mice possess loxP sites flanking exon 1 of the Kdm2b isoform 2 gene. This strain may be useful for studying the role of DNA methylation during embryonic development.
Timothy H. Bestor, Columbia University
Fbxl10-2flox mice possess loxP sites flanking exon 1 of the lysine (K)-specific demethylase 2B (Kdm2b) isoform 2 gene (also termed Fbxl10-2). This region corresponds to intron 12 in the Kdm2b isoform 1 gene (Fbxl10-1). FBXL10-2 is a F-box protein occupying every unmethylated CpG-dense promoters; FBXL10-2 is composed by a CXXC motif, a PHD motif, a leucine rich region and a F-Box domain. FBXL10 has also been reported to be an oncogene and a tumor suppressor in leukemia. Complete inactivation of the Fbxl10 gene leads to methylation only of promoters that are co-occupied by both FBXL10 and Polycomb repressive complexes (PRCs). Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 1 of Fbxl10-2 deleted in the cre-expressing tissues. Cre-recombination does not affect Fbxl10-1 gene expression.
A targeting vector was designed to insert loxP sites flanking exon 1 of the lysine (K)-specific demethylase 2B (Kdm2b) isoform 2 gene. A frt-flanked neomycin resistance (neo) cassette was inserted downstream of the second loxP site. This region corresponds to intron 12 in the Kdm2b isoform 1 gene. The construct was electroporated into C57bl6/129sv hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred with ROSA26:FLPe mice on a C57BL/6 background to delete the neo cassette. Offspring were bred to FVB/NJ mice for at least 8 generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize FVB/NJ oocytes (Stock No. 001800).
|Allele Name||targeted mutation 1.1, Timothy H Bestor|
|Allele Type||Targeted (Conditional ready (e.g. floxed))|
|Gene Symbol and Name||Kdm2b, lysine (K)-specific demethylase 2B|
|Strain of Origin||C57BL/6 x 129/Sv|
|Molecular Note||The targeting vector is designed to insert a loxP site upstream of exon 1 (including the alternative internal promoter) followed by a second loxP site and an FRT-flanked neomycin resistance (neo) cassette downstream of exon 1 of isoform 2. This region corresponds to intron 12 in the Kdm2b isoform 1 gene. Flp-mediated recombination removed the FRT-flanked neo cassette.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Fbxl10-2flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #029345 in your Materials and Methods section.