The targeted <i>Akna</i> gene encodes a transcription factor that upregulates CD40 receptor expression and is involved in secondary immune response.  A mutation in this gene has been associated with an increased risk of cervical cancer.
These knock-out mice carry a mutation in which a PGK-neo cassette replaced exons 19 through 21, which encode putative C-terminus AT-hook-like motif (ALM) sequence.  A truncated gene product (mRNA) of the theorectical ALM sequence is detected by qRT-PCR analysis.  

Homozygous neonates are smaller than wildtype controls and fail to thrive with most dying by postnatal day 10.  Surviving homozygotes are very susceptible to inflammation induced by pathogen.  Systemic inflammation with notable involvement of the lungs is detected in homozygous animals.  Although alveolar morphology is normal at postnatal days 1 to 5, by postnatal day 7, homozygotes exhibit enlarged alveolar airspace.  An increased number of neutrophils are found in bone marrow and peripheral blood.
Mice that are heterozygous for the targeted mutation are viable and fertile.
During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background.


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This <i>Akna</i> knockout strain has exons 19 through 21 deleted and may be useful in studies of secondary immune response.

A minimum of two mice must be ordered. Additional mice will be charged on a per-mouse basis. Click the “Details” button to learn more.

Our minimum guarantee for cryorecovery orders is 2 carriers. To ensure that your colony can be rapidly expanded upon arrival, additional carriers may be added to your order.