This Col1a2 Cre-ER mutant mouse strain carries a transgene with tamoxifen inducible Cre recombinase and may be useful for generating fibroblast specific targeted mutants for studies of fibrotic diseases, wound healing and connective tissue physiology, as well as fibroblast lineage fate mapping.
Benoit de Crombrugghe, UT MD Anderson Cancer Center
Christopher Denton, University College London
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
These transgenic mice express a tamoxifen-inducible Cre recombinase driven by the mouse Col1a2, collagen, type I, alpha 2, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions of the flanked sequence. Tamoxifen administration in double mutant mice, carrying this transgene and a beta-galactosidase reporter, induces Cre recombination in dermal and visceral fibroblasts. The Donating Investigator reports that homozygous mice do not breed well. Hemizygous mice are viable and fertile.
Of note, The Jackson Laboratory live colony of Stock No. 029235 shows transgene carrier and noncarrier mice exhibit white belly spots [November 2019].
Col1a2-CreER transgenic mice will also be available on a C57BL/6J congenic background (Stock No. 029567).
A transgenic construct containing sequence encoding cre/Esr1, an IRES segment and human placental alkaline phosphatase, under the control of the regulatory elements of the mouse Col1a2, collagen, type I, alpha 2, gene (6 kb of the upstream 5' flanking region upstream of the transcription start site and endogenous minimal promoter) was injected into fertilized B6D2F1/J mouse eggs. Founder line 7 was subsequently established. Upon arrival, sperm was cryopreserved. To establish a live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Seven of the 27 markers throughout the genome are segregating, suggesting an incomplete backcross. Also, all 5 markers that discern C57BL/6J from C57BL/6N were found to be segregating.
Expressed Gene | cre/ER, cre inducible estrogen receptor, bacteriophage P1/Human |
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Site of Expression | Col1a2 enhancer drives fibroblast-specific expression of a cre/ERT fusion followed by an IRES-human placental alkaline-phosphatase reporter. |
Allele Name | transgene insertion 7, Christopher P Denton |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | Col1a2 Cre-ER; Col1a2-Cre-ER(T); Col1a2CreERT; Tg(Col1a2-cre/ERT)7Cpd; Tg(Col1a2-cre/ESR1)71Pcn; Tg(Col1a2-cre/ESR1)7Cpd |
Gene Symbol and Name | Tg(Col1a2-cre/ERT,-ALPP)7Cpd, transgene insertion 7, Christopher P Denton |
Gene Synonym(s) | |
Promoter | Col1a2, collagen, type I, alpha 2, mouse, laboratory |
Expressed Gene | cre/ER, cre inducible estrogen receptor, bacteriophage P1/Human |
Site of Expression | Col1a2 enhancer drives fibroblast-specific expression of a cre/ERT fusion followed by an IRES-human placental alkaline-phosphatase reporter. |
Strain of Origin | (C57BL/6 x DBA/2)F2 |
Chromosome | UN |
Molecular Note | The Col1a2 enhancer was used to drive fibroblast-specific expression of a cre/ERT fusion followed by an IRES-human placental alkaline-phosphatase reporter. Two lines were created (lines 7 and 8) and line 7 was used in subsequent analysis. |
Mutations Made By | Christopher Denton, University College London |
When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator reports that homozygotes do not breed well.
Of note, The Jackson Laboratory live colony of Stock No. 029235 shows transgene carrier and noncarrier mice exhibit white belly spots [November 2019].
When using the Col1a2-CreER mouse strain in a publication, please cite the originating article(s) and include JAX stock #029235 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Col1a2-cre/ERT,-ALPP)7Cpd |
Frozen Mouse Embryo | STOCK Tg(Col1a2-cre/ERT -ALPP)7Cpd/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Col1a2-cre/ERT -ALPP)7Cpd/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Col1a2-cre/ERT -ALPP)7Cpd/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Col1a2-cre/ERT -ALPP)7Cpd/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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