c-Kitlox66-71 is a Cre-recombinase conditional knockout/eGFP-reporter allele - allowing cre-inducible c-Kit deletion and concomitant labeling of the c-Kit-deficient cells with eGFP. 
 Prior to Cre recombination, the c-Kitlox66-71 allele will transcribe a full-length c-Kit transcript, and no eGFP expression is reported. 
 Exposure to Cre recombinase will invert the fragment bordered by the inward-facing lox71 and lox66 sites (lox71-SA-IRES-eGFP-2pA-lox66) into sense orientation; creating the eGFP-expressing c-Kit knockout allele (c-Kit&Delta;GFP). Specifically, the transcriptional stops upstream of the exons encoding the transmembrane and kinase domains of c-Kit result in a truncated protein and loss of c-Kit cell surface expression. The splice-acceptor-IRES sequences lead to eGFP expression in c-Kit-expressing cells (direct fluorescence). The donating investigator reports eGFP direct fluorescence is observable by flow cytometry, but weak by imaging. These mice are useful for imaging of c-Kit-deficient cells in vivo as well as facilitating isolation of these cells in vitro.

c-Kitlox66-71 is a Cre-recombinase conditional knockout/eGFP-reporter allele - allowing cre-inducible c-Kit deletion and concomitant labeling of the c-Kit-deficient cells with eGFP. These mice are useful for imaging of c-Kit-deficient cells in vivo as well as facilitating isolation of these cells in vitro.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. For additional information and to purchase, please visit the MMRRC site.