This Gadd45b knock-out strain is useful in studies of memory and synaptic plasticity, and apoptosis.
Dan Liebermann, Temple University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Gadd45b | growth arrest and DNA-damage-inducible 45 beta |
The targeted Gadd45b gene encodes a sensor of physiological and environmental stress that mediates activation of the p38/JNK pathway. These mice carry a knock-out allele of the Gadd45b gene in which the entire protein coding region and flanking sequence has been replaced by a NEO cassette. No gene product (mRNA) is detected by Northern blot analysis of bone marrow cells from homozygotes.
Mice that are homozygous for the targeted mutation are viable and fertile. Bone marrow cells isolated from homozygotes are more sensitive to UV radiation, daunorubicin, or VP-16, exhibiting increased apoptosis. Homozygotes have slightly increased liver mass, but smaller hepatocytes, with a delayed drug-induced hyperplasia response compared to wildtype controls. Knock-out mice exhibit enhanced persisting memory tasks of motor performance, aversive conditioning and spatial navigation.
A targeting vector containing a NEO cassette (in antisense transcriptional orientation) was used to disrupt 301 bp of the proximal promoter, the 5' untranslated region of the mRNA, the entire protein coding region and 425 bp of the 3' untranslated region of the targeted Gadd45b gene. The construct was electroporated into 129X1/SvJ derived ESVJ-1183 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.
The resulting chimeric male animals were crossed to C57BL/6 female mice. Heterozygotes were intercrossed to generate homozygotes.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Allele Name | targeted mutation 1, Dan A Liebermann |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Gadd45b- |
Gene Symbol and Name | Gadd45b, growth arrest and DNA-damage-inducible 45 beta |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 10 |
Molecular Note | 301 bp of the proximal promoter, the 5' untranslated region of the mRNA, the entire protein coding region and 425 bp of the 3' untranslated region were replaced by a 1.9 kb neo cassette (transcription of neo is antisense) from the plasmid pGT-N29 via homologous recombination. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Gadd45b knock-out mouse strain in a publication, please cite the originating article(s) and include JAX stock #029141 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gadd45b<tm1Daa> |
Frozen Mouse Embryo | B6;129X1-Gadd45b<tm1Daa>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129X1-Gadd45b<tm1Daa>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129X1-Gadd45b<tm1Daa>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129X1-Gadd45b<tm1Daa>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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