Withdrawal Seizure-Resistant, replicate 1 and 2 (WSR1, WSR2) mice were selectively bred to exhibit low alcohol withdrawal convulsion severity. Used in conjunction with the Withdrawal Seizure-Prone, replicate 1 and 2 (WSP1, WSP2) and control replicate 1 and 2 lines (WSC1, WSC2) they may be useful for studying the genetic and neurobiological basis of alcohol withdrawal severity.
John C Crabbe, Oregon Health & Science University
The Withdrawal Seizure-Resistant replicate lines (WSR1 and WSR2) were selectively bred for low alcohol withdrawal convulsion severity following 72hr of continuous chronic ethanol vapor inhalation combined with the administration of pyrazole to inhibit ethanol metabolism and stabilize blood alcohol levels. Mice were evaluated for responsiveness to handling-induced convulsions.
In parallel, Withdrawal Seizure-Prone replicate lines (WSP1 and WSP2) were selectively bred for high alcohol withdrawal convulsion severity after an identical regimen of chronic exposure to ethanol and show withdrawal handling-induced convulsions. Both WSR replicate lines exhibit seizures approximately 10-fold less severe than the WSP replicate lines.
Control lines (WSC1, WSC2) were generated by random matings within each replicate line.
Each line consists of five families that are bred to each other by rotation. The effect is to fix alleles conferring low seizure withdrawal severity in the homozygous state, to eliminate alleles conferring high withdrawal seizure severity and to allow the rest of the genome to segregate randomly.
Used in conjunction with the Withdrawal Seizure-Prone, replicate 1 and 2 (WSP1, WSP2) and the control lines (WSC1, WSC2) they may be useful for studying the genetic and neurobiological basis of alcohol withdrawal severity.
Withdrawal Seizure-Resistant, replicate 1 (WSR1) mice were selectively bred for low alcohol withdrawal convulsion severity after 72hr of continuous chronic ethanol vapor inhalation. The founder stock for selective breeding was the HS/Ibg heterogeneous stock that was developed by Gerald McClearn and colleagues (The Institute for Behavioral Genetics, Boulder, Colorado) as an 8-way cross of the following inbred strains obtained from the Cancer Research Genetics Laboratory at UC-Berkeley: A, AKR, BALB/c, C3H/2, C57BL, DBA/2, Is/Bi, and RIII.
Eighteen matings of Generation 30 HS/Ibg mice were randomly divided into 2 groups of 9 families each. These generation 31 mice became the foundation (So) population for selection based on alcohol withdrawal severity. One group of 9 familes was designated Replicate 1, the other Replicate 2. To establish the Withdrawal Seizure-Resistant Replicate 1 (WSR1) line the lowest scoring male and female from each family were selected. Selected progeny from each family were then mated using a rotational system – female from Family 1 mated to male from Family 2, female from Family 2 mated to male from Family 3, etc. Two to six reserve families were generated to provide back up. The selection process was repeated for 26 generations. Relaxed breeding (random selection of breeders from within each family) has continued for approximately 100 generations. The MMRRC imported a acombination of the 9 families to form the WSR1 line in 2016.
Currently there are no related genes or alleles for this strain.
The Withdrawal Seizure-Resistant, replicate 1 line consists of 5 families that were combined to form the WSR1 line. This line is maintained as a closed mating system.
When using the Withdrawal Seizure-Resistant, replicate 1 mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #41487 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.