Overview

Also Known As:MRAP2 KO

Mrap2 KO mice have a frt-flanked β-gal gene and neo cassette preventing widespread expression of the melanocortin 2 receptor accessory protein 2 (Mrap2) gene. Exon 4 is flanked by loxP sites making this strain useful for conditional deletion as well. These mice may be suitable for applications related to energy homeostasis and food intake.

Donating Investigator

Julien A Sebag, University of Iowa

Genetic overview

Genetic Background	Generation

Mrap2^{tm1a(EUCOMM)Wtsi}

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), Reporter, Null/Knockout)	Mrap2	melanocortin 2 receptor accessory protein 2

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Research Applications

Research Tools
Diabetes and Obesity Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mrap2 KO mice have a β-galactosidase gene and a neo cassette preventing expression of the melanocortin 2 receptor accessory protein 2 (Mrap2) gene. Also, loxP sites flank exon 4. MRAP2 is a G-Protein Coupled Receptor accessory protein that regulates energy homeostasis and food intake through receptors such as melanocortin-4 receptor (MC4R) and prokineticin receptor 1 (PKR1). Homozygous Mrap2 KO mice are viable, fertile, and severely obese.

A conditional ready floxed allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in the deletion of exon 4. If cre expression occurs without flp expression, a β-galactosidase reporter knockout mouse will be created.

Development

C57BL/6N-A^tm1Brd-derived JM8A3.N1 embryonic stem (ES) cells containing a targeted mutation of the melanocortin 2 receptor accessory protein 2 (Mrap2) gene were obtained from The European Conditional Mouse Mutagenesis Program (EUCOMM). The mutant allele has, from 5’ to 3’, a FRT site, a β-galactosidase and polyA sequence, a loxP site, a neomycin resistance (neo) cassette, a second FRT site, and loxP sites flanking exon 4. Targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6NJ oocytes (Stock No. 005304).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Suggestions

Approximate Controls

005304 C57BL/6NJ

Additional Information

Considerations for Choosing Controls

Selected References

Chaly AL; Srisai D; Gardner EE; Sebag JA. 2016. The Melanocortin Receptor Accessory Protein 2 promotes food intake through inhibition of the Prokineticin Receptor-1. Elife 5PubMed: 26829592 MGI: J:230718

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Genetics

Mrap2^{tm1a(EUCOMM)Wtsi}

Allele Symbol: Mrap2^{tm1a(EUCOMM)Wtsi}

Allele Name	targeted mutation 1a, Wellcome Trust Sanger Institute
Allele Type	Targeted (Conditional ready (e.g. floxed), Reporter, Null/Knockout)
Allele Synonym(s)
Gene Symbol and Name	Mrap2, melanocortin 2 receptor accessory protein 2
Gene Synonym(s)
Strain of Origin	C57BL/6N-A^tm1Brd
Chromosome	9
Molecular Note	The L1L2_Bact_P cassette was inserted at position 87175333 of Chromosome 9 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 87176135. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Tissue/Cell Markers: Cre-lox System
- Cre-lox System
  - loxP-flanked Sequences
Diabetes and Obesity Research
- Obesity Without Diabetes

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Mrap2^{tm1a(EUCOMM)Wtsi}/Mrap2^{tm1a(EUCOMM)Wtsi}
C57BL/6N-Mrap2/Wtsi

adipose tissue phenotype

abnormal adipose tissue amount

increased total body fat amount

growth/size/body region phenotype

decreased lean body mass

increased lean body mass

abnormal lean body mass

hematopoietic system phenotype

abnormal red blood cell distribution width

decreased B cell number

increased leukocyte cell number

abnormal leukocyte cell number

increased red blood cell distribution width

decreased NK cell number

increased monocyte cell number

homeostasis/metabolism phenotype

abnormal circulating alkaline phosphatase level

increased circulating alkaline phosphatase level

increased circulating calcium level

increased circulating glycerol level

increased circulating HDL cholesterol level

increased circulating triglyceride level

increased circulating LDL cholesterol level

increased circulating amylase level

increased circulating cholesterol level

immune system phenotype

decreased B cell number

abnormal leukocyte cell number

increased leukocyte cell number

decreased NK cell number

increased monocyte cell number

skeleton phenotype

abnormal bone mineralization

decreased bone mineral content

References

Chaly AL; Srisai D; Gardner EE; Sebag JA. 2016. The Melanocortin Receptor Accessory Protein 2 promotes food intake through inhibition of the Prokineticin Receptor-1. Elife 5PubMed: 26829592 MGI: J:230718

Additional - Mrap2^{tm1a(EUCOMM)Wtsi} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Mrap2 Alternate1
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Citation
When using the MRAP2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #029050 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Not-For-Profit & Academic Pricing

Service/Product	Description	Price
	Heterozygous for Mrap2<tm1a(EUCOMM)Wtsi>

Related Products and Services

Frozen Mouse Embryo	C57BL/6-Mrap2<tm1a(EUCOMM)Wtsi>/JsbgJ	$2595.00
Frozen Mouse Embryo	C57BL/6-Mrap2<tm1a(EUCOMM)Wtsi>/JsbgJ	$2595.00
Frozen Mouse Embryo	C57BL/6-Mrap2<tm1a(EUCOMM)Wtsi>/JsbgJ	$3373.50
Frozen Mouse Embryo	C57BL/6-Mrap2<tm1a(EUCOMM)Wtsi>/JsbgJ	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Not Available to Companies or for Commercial Use

Use of MICE only available to non-profit entities.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:MRAP2 KO

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Mrap2tm1a(EUCOMM)Wtsi

Allele Symbol: Mrap2tm1a(EUCOMM)Wtsi

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Mrap2tm1a(EUCOMM)Wtsi/Mrap2tm1a(EUCOMM)WtsiC57BL/6N-Mrap2/Wtsi

adipose tissue phenotype

growth/size/body region phenotype

hematopoietic system phenotype

homeostasis/metabolism phenotype

immune system phenotype

skeleton phenotype

References

Additional - Mrap2tm1a(EUCOMM)Wtsi related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Not-For-Profit & Academic Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Mrap2^{tm1a(EUCOMM)Wtsi}

Allele Symbol: Mrap2^{tm1a(EUCOMM)Wtsi}

Genotype: Mrap2^{tm1a(EUCOMM)Wtsi}/Mrap2^{tm1a(EUCOMM)Wtsi}
C57BL/6N-Mrap2/Wtsi

Additional - Mrap2^{tm1a(EUCOMM)Wtsi} related