These Id4 knock-in/knock-out mice exhibit reduced brain size, impaired cell cycle transition in early neural stem cells, reduced body fat, resistance to high fat diet and impaired adipocyte differentiation. This strain may be useful for studying ID4 as a regulator of neurogenesis and adipogenesis.
Giles Duffield, University of Notre Dame
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Null/Knockout) | Id4 | inhibitor of DNA binding 4 |
ID4 (inhibitor of DNA binding 4) is a transcriptional regulator containing a helix-loop-helix domain and lacking a DNA binding domain. Unique among the ID proteins, ID4 is involved in the regulation of neural stem cell differentiation and proliferation. The Id4 knockout/knockin allele has a GFP reporter and a PGK-neo cassette replacing exons 1-2 of the gene. Uniform GFP expression is observed in all cortical areas by E12.5. Mice homozygous for this null allele exhibit reduced brain size from E11.5, specifically a 30% reduction occurs in the ventricular surface of the telencephalon affecting dorsomedial progenitor zones. Impairment of G1-S cell cycle transition and premature differentiation in early neural stem cells results in reduced brain size in these mice. Like ID2, loss of ID4 affects adipogenesis. Homozygous mice have 50% less body fat weight and adipocyte size is reduced by half. Following 6 weeks on high fat diet, mice gain significantly less weight than wild-type littermates. This strain may be useful for studying ID4 as a regulator of neurogenesis and adipogenesis.
A targeting vector containing the GFP (green fluorescent protein) coding regions and the neomycin resistance cassette was used to replace exons 1 and 2 through homologous recombination. The construct was electroporated into electroporated into 129X1/SvJ-derived JM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and then backcrossed to C57BL/6J in excess of 5 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Site of Expression | GFP is expressed in the brain. |
Allele Name | targeted mutation 1, Mark A Israel |
---|---|
Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Id4-; Id4GFP |
Gene Symbol and Name | Id4, inhibitor of DNA binding 4 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP is expressed in the brain. |
Strain of Origin | 129X1/SvJ |
Chromosome | 13 |
Molecular Note | GFP and a neomycin-resistance gene replaced exons 1 and 2 via homologous recombination. GFP expression was observed in the brain. |
While maintaining a live colony, these mice are bred as heterozygotes. The Donating Investigator has not attempted to breed homozygotes. Heterozygote x heterozygote crosses produce fewer homozygotes than expected (14% vs. 25%).
When using the Id4- mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #41569 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.