NOD-PerL transgenic mice express the Igk light chain gene of islet-derived peripherin-specific B cell hybridoma H280. They have accelerated T1D onset with increased insulitis. These mice may be useful when studying the effect of neuronal autoimmunity on T1D development.
Dr. David Serreze, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
NOD-PerL mice express the Igk light chain gene of islet-derived peripherin-specific B cell hybridoma H280. Approximately 50% of pancreatic islet associated B lymphocytes in NOD mice recognize the autoantigen peripherin. Peripherin is expressed widely in neuronal cell bodies and axons of the peripheral and central nervous system. Expression of peripherin also occurs in the peri-insular areas of postnatal mice NOD. The H280 clone was chosen since it was isolated from the islets of a recent T1D onset NOD mouse, exhibited strong peripherin binding, and neither its heavy or light chain demonstrate any evidence of affinity maturation.
Hemizygous NOD-PerL mice exhibit greatly accelerated Type 1 diabetes (T1D) onset, at a frequency of between 90-95% by 20 weeks of age. While control NOD/ShiLtJ mice (Stock No. 001976) T1D develops at a frequency of between 80-90% by 30-40 weeks of age. Insulitis development is also accelerated in this strain compared to controls.
When NOD-PerL mice are crossed with NOD-PerH mice, expressing the peripherin-specific B cell hybridoma H280 Ighm (IgM) and Ighd (IgD) heavy chain genes (Stock No. 028930), resulting NOD-PerIg mice have further accelerated T1D development and insulitis compared to the separate transgenic lines.
The PerL transgenic construct, containing the Igk light chain gene of islet-derived peripherin-specific B cell hybridoma H280, was injected into fertilized NOD/LtDvs oocytes by Dr. Dave Serreze (The Jackson Laboratory). Progeny of the NOD-PerL transgenic founder 934 were bred to NOD/ShiLtDvs for 2 generations. Male and female carriers were sent to The Jackson Laboratory Repository to establish a colony.
Expressed Gene | Igk, immunoglobulin kappa chain complex, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 934, David V Serreze |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | PerL |
Gene Symbol and Name | Tg(IgkH280)934Dvs, transgene insertion 934, David V Serreze |
Gene Synonym(s) | |
Promoter | Igk, immunoglobulin kappa chain complex, mouse, laboratory |
Expressed Gene | Igk, immunoglobulin kappa chain complex, mouse, laboratory |
Strain of Origin | NOD/ShiLtDvs |
Chromosome | UN |
Molecular Note | The transgenic construct contains the Igk light chain gene of islet-derived peripherin-specific B cell hybridoma H280. |
When maintaining a live colony, homozygous mice may be bred together.
When using the NOD-PerL mouse strain in a publication, please cite the originating article(s) and include JAX stock #028931 in your Materials and Methods section.
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