NOD-PerH mice express Ighm (IgM) and Ighd (IgD) heavy chain genes of islet-derived peripherin-specific B cell hybridoma H280. Approximately 50% of pancreatic islet associated B lymphocytes in NOD mice recognize the autoantigen peripherin.
Peripherin is expressed widely in neuronal cell bodies and axons of the peripheral and central nervous system. Expression of peripherin also occurs in the peri-insular areas of postnatal NOD mice. The H280 clone was chosen since it was isolated from the islets of a
recent T1D onset NOD mouse, exhibited strong peripherin binding, and neither its heavy or light chain demonstrate any evidence of affinity maturation.
Hemizygous NOD-PerH mice exhibit greatly accelerated Type 1 diabetes (T1D) onset, at a frequency of between 90-95% by 20 weeks of age. While control NOD/ShiLtJ mice (Stock No. 001976) develop T1D at a frequency of between 80-90% by 30-40 weeks of age. Insulitis development is also accelerated in this strain compared to controls.
When NOD-PerH mice are crossed with NOD-PerL mice, expressing the peripherin-specific B cell hybridoma H280 Igk light chain (Stock No. 028931), resulting NOD-PerIg mice have further accelerated T1D development and insulitis compared to the separate transgenic lines.
