Overview

Also Known As:TLR5 null (TLR5^-)

The TLR5 null allele (TLR5^-) mice have exon 4 of the toll-like receptor 5 gene deleted. These knock-out mice may be useful in studying TLR5 recognition of pathogens, cytokine secretion and activation of innate immune responses.
Of note, a C57BL/6-congenic TLR5 floxed exon 4 allele (TLR5^fl) is also available as Stock No. 028599.

Donating Investigator

Andrew T Gewirtz, Georgia State University

Benoit Chassaing, Georgia State University

Genetic overview

Genetic Background	Generation

Tlr5^tm1.2Gewr

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Tlr5	toll-like receptor 5

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Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

The Tlr5 gene encodes toll-like receptor 5, which has a fundamental role in bacteria recognition and activation of innate immune responses. TLR5 is the receptor for bacterial flagellin, acting via MYD88 and TRAF6; leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

The TLR5 null allele (TLR5^-) has exon 4 of the toll-like receptor 5 gene deleted throughout the germline. No functional protein is expressed from this knockout allele. Homozygous mice (TLR5^-/-) are expected to exhibit increased levels of flagellated bacteria in the intestine, that will promote low-grade inflammation, metabolic syndrome and colitis.

In response to scientific interest, we may offer floxed alleles as well as their resulting germline-knockout alleles. The knockout phenotype described above was for C57BL/6-congenic TLR5^fl mice (Stock No. 028599) bred to C57BL/6-congenic mice expressing non-germline Cre recombinase. It should be noted that the phenotype of these C57BL/6-congenic TLR5 null mice (Stock No. 028909) could vary from that expected/inferred from other similar models. We may modify this strain description if necessary as published results become available.

Development

The TLR5 null allele (TLR5^-) was generated by breeding TLR5^fl mice with the germline cre-expressing Sox2-Cre mouse line, and then removing the Sox2-Cre allele. Specific details below.

The TLR5^fl strain was created by Dr. Andrew T. Gewirtz and Benoit Chassaing (Georgia State University) to have loxP sites flanking exon 4 of toll-like receptor 5 gene (Tlr5) on chromosome 1. TLR5^fl mice on a C57BL/6-congenic background arrived at The Jackson Laboratory Repository in 2016 as Stock No. 028599. Upon arrival, some floxed animals were bred at least one additional generation to C57BL/6NJ inbred mice (Stock No. 005304). Next, they were bred to the germline cre-expressing, C57BL/6-congenic Sox2-Cre strain (Stock No. 008454). The resulting animals with the TLR5 null allele (TLR5^-) were bred together or to wildtype (and the Sox2-Cre transgene was removed). The C57BL/6-congenic TLR5^- colony is Stock No. 028909. The genetic background is expected to be a mix of C57BL/6J and C57BL/6N.

Control Suggestions

Wild-type from the colony

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Chassaing B; Ley RE; Gewirtz AT. 2014. Intestinal epithelial cell toll-like receptor 5 regulates the intestinal microbiota to prevent low-grade inflammation and metabolic syndrome in mice. Gastroenterology 147(6):1363-77.e17PubMed: 25172014 MGI: J:229155

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Genetics

Tlr5^tm1.2Gewr

Allele Symbol: Tlr5^tm1.2Gewr

Allele Name	targeted mutation 1.2, Andrew Gewirtz
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)
Gene Symbol and Name	Tlr5, toll-like receptor 5
Gene Synonym(s)
Strain of Origin	C57BL/6NTac
Chromosome	1
Molecular Note	A targeting vector was designed to place a loxP site upstream of exon 4, and a FRT::loxP::Neo::FRT::loxP cassette inserted 211 base pairs downstream of exon 4. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 4 floxed. Cre-mediated recombination removed exon 4.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - Tlr deficiency
  - multiple immune defects
  - Inflammatory bowel disease
- Inflammation
  - Tlr deficiency
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
  - genes regulating susceptibility to infectious disease and endotoxin
  - Tlr deficiency
- Growth Factors/Receptors/Cytokines
- Intracellular Signaling Molecules
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - genes regulating susceptibility to infectious disease and endotoxin
- Infectious Disease

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Tlr5^tm1.2Gewr/Tlr5^tm1.2Gewr
B6(Cg)-Tlr5/J

mammalian phenotype

no phenotypic analysis

References

Chassaing B; Ley RE; Gewirtz AT. 2014. Intestinal epithelial cell toll-like receptor 5 regulates the intestinal microbiota to prevent low-grade inflammation and metabolic syndrome in mice. Gastroenterology 147(6):1363-77.e17PubMed: 25172014 MGI: J:229155

Additional - Tlr5^tm1.2Gewr related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Tlr5
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Alternatively, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Citation
When using the TLR5 null (TLR5^-) mouse strain in a publication, please cite the originating article(s) and include JAX stock #028909 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for Tlr5<tm1.2Gewr>

Related Products and Services

Frozen Mouse Embryo	B6(Cg)-Tlr5<tm1.2Gewr>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6(Cg)-Tlr5<tm1.2Gewr>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6(Cg)-Tlr5<tm1.2Gewr>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6(Cg)-Tlr5<tm1.2Gewr>/J Frozen Embryo	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Also Known As:TLR5 null (TLR5-)

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Tlr5tm1.2Gewr

Allele Symbol: Tlr5tm1.2Gewr

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tlr5tm1.2Gewr/Tlr5tm1.2GewrB6(Cg)-Tlr5/J

mammalian phenotype

References

Additional - Tlr5tm1.2Gewr related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As:TLR5 null (TLR5^-)

Tlr5^tm1.2Gewr

Allele Symbol: Tlr5^tm1.2Gewr

Genotype: Tlr5^tm1.2Gewr/Tlr5^tm1.2Gewr
B6(Cg)-Tlr5/J

Additional - Tlr5^tm1.2Gewr related