The TLR5 null allele (TLR5-) mice have exon 4 of the toll-like receptor 5 gene deleted. These knock-out mice may be useful in studying TLR5 recognition of pathogens, cytokine secretion and activation of innate immune responses.
Of note, a C57BL/6-congenic TLR5 floxed exon 4 allele (TLR5fl) is also available as Stock No. 028599.
Andrew T Gewirtz, Georgia State University
Benoit Chassaing, Georgia State University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Tlr5 | toll-like receptor 5 |
The Tlr5 gene encodes toll-like receptor 5, which has a fundamental role in bacteria recognition and activation of innate immune responses. TLR5 is the receptor for bacterial flagellin, acting via MYD88 and TRAF6; leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
The TLR5 null allele (TLR5-) has exon 4 of the toll-like receptor 5 gene deleted throughout the germline. No functional protein is expressed from this knockout allele. Homozygous mice (TLR5-/-) are expected to exhibit increased levels of flagellated bacteria in the intestine, that will promote low-grade inflammation, metabolic syndrome and colitis.
In response to scientific interest, we may offer floxed alleles as well as their resulting germline-knockout alleles. The knockout phenotype described above was for C57BL/6-congenic TLR5fl mice (Stock No. 028599) bred to C57BL/6-congenic mice expressing non-germline Cre recombinase. It should be noted that the phenotype of these C57BL/6-congenic TLR5 null mice (Stock No. 028909) could vary from that expected/inferred from other similar models. We may modify this strain description if necessary as published results become available.
The TLR5 null allele (TLR5-) was generated by breeding TLR5fl mice with the germline cre-expressing Sox2-Cre mouse line, and then removing the Sox2-Cre allele. Specific details below.
The TLR5fl strain was created by Dr. Andrew T. Gewirtz and Benoit Chassaing (Georgia State University) to have loxP sites flanking exon 4 of toll-like receptor 5 gene (Tlr5) on chromosome 1. TLR5fl mice on a C57BL/6-congenic background arrived at The Jackson Laboratory Repository in 2016 as Stock No. 028599. Upon arrival, some floxed animals were bred at least one additional generation to C57BL/6NJ inbred mice (Stock No. 005304). Next, they were bred to the germline cre-expressing, C57BL/6-congenic Sox2-Cre strain (Stock No. 008454). The resulting animals with the TLR5 null allele (TLR5-) were bred together or to wildtype (and the Sox2-Cre transgene was removed). The C57BL/6-congenic TLR5- colony is Stock No. 028909. The genetic background is expected to be a mix of C57BL/6J and C57BL/6N.
Allele Name | targeted mutation 1.2, Andrew Gewirtz |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Tlr5, toll-like receptor 5 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6NTac |
Chromosome | 1 |
Molecular Note | A targeting vector was designed to place a loxP site upstream of exon 4, and a FRT::loxP::Neo::FRT::loxP cassette inserted 211 base pairs downstream of exon 4. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 4 floxed. Cre-mediated recombination removed exon 4. |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Alternatively, homozygous mice may be bred together.
When using the TLR5 null (TLR5-) mouse strain in a publication, please cite the originating article(s) and include JAX stock #028909 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Tlr5<tm1.2Gewr> |
Frozen Mouse Embryo | B6(Cg)-Tlr5<tm1.2Gewr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Tlr5<tm1.2Gewr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Tlr5<tm1.2Gewr>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Tlr5<tm1.2Gewr>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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