IL-21-Cre mice express codon-optimized Cre recombinase in IL-21 producing CD4+ T cell populations and may be useful in generating reporter mice that allow for the characterization and tracing of the development of IL-21 expressing cells.
Gerd Mueller, Max-Delbrueck-Center for Molecular Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
IL-21-Cre mice express a codon optimized Cre recombinase (iCre) under control of the mouse interleukin 21 (Il21) promoter. IL-21 is a cytokine produced by CD4+ T cell populations, in particular T follicular helper (TFH) cells, TH17 cells, and natural killer T (NKT) cells. IL-21 exerts numerous immune-enhancing and immune-regulatory functions, thus playing a critical role in the initiation and control of both innate and adaptive immune responses. It acts on a broad range of cell types including, but not limited to, T cells, B cells, macrophages, monocytes, and dendritic cells by modulating proliferation, differentiation, phagocytosis, apoptosis, and the production of other cytokines. IL-21-Cre mice mice may be particularly useful for the generation of reporter mice that allow for the characterization and tracing of the development of IL-21 expressing cells such as follicular T helper (Tfh) cells. Hemizygous mice are viable and fertile. The donating investigator did not attempt to make a homozygous colony.
Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (RP24-291E22) containing the entire murine Il21 (interleukin 21) gene. This BAC was modified to insert a codon optimized Cre recombinase gene (iCre) downstream of the Il21 promoter, replacing exons 1-2. The modified BAC was microinjected into fertilized C57BL/6 oocytes. IL-21-Cre founder line G was established and then subsequently maintained by breeding transgenic mice with C57BL/6 mice. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Follicular helper T-cells. |
Allele Name | transgene insertion G, Martin Lipp |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | IL21-Cre |
Gene Symbol and Name | Tg(Il21-cre)GLipp, transgene insertion G, Martin Lipp |
Gene Synonym(s) | |
Promoter | Il21, interleukin 21, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Follicular helper T-cells. |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | The transgenic vector was constructed using the RP24-291E22 BAC from CHORI containing the entire murine Il21 gene. The iCre replaced exons 1-2 of Il21 downstream of the promoter. Founder mice line G was established and maintained in breeding transgenic mice. |
When maintaining a live colony hemizygous mice may be bred together. The donating investigator did not attempt to make a homozygous colony.
When using the IL21-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #028840 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Il21-cre)GLipp |
Frozen Mouse Embryo | C57BL/6-Tg(Il21-cre)GLipp/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Il21-cre)GLipp/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Il21-cre)GLipp/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Il21-cre)GLipp/J | $3373.50 |
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