This Becn1, beclin 1, knock-out strain is useful in applications related to the regulation of autophagy, tumorigenesis, tumor progression and neurodegeneration.
Zhenyu Yue, Mount Sinai School of Medicine
Beclin 1, encoded by the Becn1 gene, plays a central role in autophagy regulation and is involved in early embryonic development, tumorigenesis, tumor progression and neurodegeneration.
These mice carry a knock-out allele of the Becn1 gene in which exon 1, exon 2, and 1.6 kb of 5’ flanking sequence has been replaced by a NEO cassette.
Mice that are heterozygous for the targeted mutation are viable and fertile. Homozygous null mice have an embryonic lethal phenotype, failing to develop past E7.5 and E8.5.
No gene product (protein) is detected by Western blot analysis of homozygous embryonic stem cells.
ES cells isolated from heterozygotes have a defective autophagic response to nutrient deprivation.
Heterozygotes aged 18 to 22 months, exhibit increased tumor incidence compared to wildtype controls. Heterozygotes display increased an immune response to respiratory viral infections.
A targeting vector containing a PGK-Neo cassette was used to disrupt exon 1, exon 2, and 1.6 kb of 5’ flanking sequence. The construct was electroporated into unspecified 129P2/OlaHsd derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission, and then intercrossed to generate homozygotes.
The mice were then backcrossed to C57BL/6 for 10 generations.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1, Nathaniel Heintz|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Becn1, beclin 1, autophagy related|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Exons 1 and 2 and 1.6 kb of upstream sequence were replaced with a neomycin selection cassette inserted by homologous recombination. Protein was undetected by Western blot analysis of homozygous mutant ES cells.|
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). Homozygotes are not viable.
When using the beclin 1 +/- mouse strain in a publication, please cite the originating article(s) and include JAX stock #028796 in your Materials and Methods section.