This Becn1, beclin 1, knock-out strain is useful in applications related to the regulation of autophagy, tumorigenesis, tumor progression and neurodegeneration.
Zhenyu Yue, Mount Sinai School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Becn1 | beclin 1, autophagy related |
Beclin 1, encoded by the Becn1 gene, plays a central role in autophagy regulation and is involved in early embryonic development, tumorigenesis, tumor progression and neurodegeneration.
These mice carry a knock-out allele of the Becn1 gene in which exon 1, exon 2, and 1.6 kb of 5’ flanking sequence has been replaced by a NEO cassette.
Mice that are heterozygous for the targeted mutation are viable and fertile. Homozygous null mice have an embryonic lethal phenotype, failing to develop past E7.5 and E8.5.
No gene product (protein) is detected by Western blot analysis of homozygous embryonic stem cells.
ES cells isolated from heterozygotes have a defective autophagic response to nutrient deprivation.
Heterozygotes aged 18 to 22 months, exhibit increased tumor incidence compared to wildtype controls. Heterozygotes display increased an immune response to respiratory viral infections.
A targeting vector containing a PGK-Neo cassette was used to disrupt exon 1, exon 2, and 1.6 kb of 5’ flanking sequence. The construct was electroporated into unspecified 129P2/OlaHsd derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission, and then intercrossed to generate homozygotes.
The mice were then backcrossed to C57BL/6 for 10 generations.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Allele Name | targeted mutation 1, Nathaniel Heintz |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Beclin- |
Gene Symbol and Name | Becn1, beclin 1, autophagy related |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 11 |
Molecular Note | Exons 1 and 2 and 1.6 kb of upstream sequence were replaced with a neomycin selection cassette inserted by homologous recombination. Protein was undetected by Western blot analysis of homozygous mutant ES cells. |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). Homozygotes are not viable.
When using the beclin 1 +/- mouse strain in a publication, please cite the originating article(s) and include JAX stock #028796 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Becn1<tm1Htz> |
Frozen Mouse Embryo | B6.129P2-Becn1<tm1Htz>/J | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Becn1<tm1Htz>/J | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Becn1<tm1Htz>/J | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Becn1<tm1Htz>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.