Withdrawal Seizure-Control, replicate 1 and 2 (WSC1, WSC2) mice were derived by random matings using the HS/Ibg heterogeneous stock. These lines serve as controls for the Withdrawal Seizure-Prone, replicate 1 and 2 (WSP1, WSP2) and Withdrawal Seizure-Resistant, replicate 1 and 2 (WSR1, WSR2) lines. WSP and WSR were selectively bred for for high and low (respectively) alcohol withdrawal convulsion severity after a regimen of chronic exposure to ethanol. These lines may be useful for studying the genetic and neurobiological basis of alcohol withdrawal severity.
John C Crabbe, Oregon Health & Science University
The Withdrawal Seizure-Control replicate lines (WSC1 and WSC2) were generated by random matings within each replicate line. These strains serve as controls for the selectively bred Withdrawal Seizure Prone (WSP) and Withdrawal Seizure Resistant (WSR) lines.
In parallel to the control lines, Withdrawal Seizure-Prone replicate lines (WSP1 and WSP2) were selectively bred for high alcohol withdrawal convulsion severity following 72hr of continuous chronic ethanol vapor inhalation combined with the administration of pyrazole to inhibit ethanol metabolism and stabilize blood alcohol levels. Both WSP replicate lines exhibit seizures approximately 10-fold more severe than the WSR and WSC replicate lines. Withdrawal Seizure-Resistant replicate lines (WSR1 and WSR2) were selectively bred for low alcohol withdrawal convulsion severity after the same regimen of chronic exposure to ethanol used for the WSP lines.
Each control line consists of five families that are bred to each other by rotation. The effect is to allow the genome to segregate randomly.
Used in conjunction with the Withdrawal Seizure-Prone, replicate 1 and 2 (WSP1, WSP2) and the Resistant lines (WSR1, WSR2) they may be useful for studying the genetic and neurobiological basis of alcohol withdrawal severity.
Withdrawal Seizure-Control, replicate 2 (WSC2) mice were derived from the HS/Ibg heterogeneous stock that was developed by Gerald McClearn and colleagues (The Institute for Behavioral Genetics, Boulder, Colorado) as an 8-way cross of the following inbred strains obtained from the Cancer Research Genetics Laboratory at UC-Berkeley: A, AKR, BALB/c, C3H/2, C57BL, DBA/2, Is/Bi, and RIII.
Eighteen matings of Generation 30 HS/Ibg mice were randomly divided into 2 groups of 9 families each. These generation 31 mice became the foundation (So) population. One group of 9 families was designated Replicate 1, the other Replicate 2. Progeny from each family were then mated using a rotational system – female from Family 1 mated to male from Family 2, female from Family 2 mated to male from Family 3, etc. Two to six reserve families were generated to provide back up. Relaxed breeding (random selection of breeders from within each family) has continued for approximately 100 generations. The MMRRC imported 5 of the 9 families, designated WSC2-1, WSC2-2, WSC2-3, WSC2-4, WSC2-5 in 2016.
Currently there are no related genes or alleles for this strain.
The Withdrawal Seizure-Control, replicate 2 line consists of 5 families that were combined to form the WSC2 line. This line is maintained as a closed mating system.
When using the Withdrawal Seizure Control 2 mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #41490 in your Materials and Methods section.