Overview

Also Known As:Withdrawal Seizure Control, Replicate 1

Withdrawal Seizure-Control, replicate 1 and 2 (WSC1, WSC2) mice were derived by random matings using the HS/Ibg heterogeneous stock. These lines serve as controls for the Withdrawal Seizure-Prone, replicate 1 and 2 (WSP1, WSP2) and Withdrawal Seizure-Resistant, replicate 1 and 2 (WSR1, WSR2) lines. WSP and WSR were selectively bred for for high and low (respectively) alcohol withdrawal convulsion severity after a regimen of chronic exposure to ethanol. These lines may be useful for studying the genetic and neurobiological basis of alcohol withdrawal severity

Donating Investigator

John C Crabbe, Oregon Health & Science University

Genetic overview

Genetic Background	Generation

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Research Applications

Research Tools
Neurobiology Research

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Details

Detailed Description

The Withdrawal Seizure-Control replicate lines (WSC1 and WSC2) were generated by random matings within each replicate line. These strains serve as controls for the selectively bred Withdrawal Seizure Prone (WSP) and Withdrawal Seizure Resistant (WSR) lines.

In parallel to the control lines, Withdrawal Seizure-Prone replicate lines (WSP1 and WSP2) were selectively bred for high alcohol withdrawal convulsion severity following 72hr of continuous chronic ethanol vapor inhalation combined with the administration of pyrazole to inhibit ethanol metabolism and stabilize blood alcohol levels. Both WSP replicate lines exhibit seizures approximately 10-fold more severe than the WSR and WSC replicate lines. Withdrawal Seizure-Resistant replicate lines (WSR1 and WSR2) were selectively bred for low alcohol withdrawal convulsion severity after the same regimen of chronic exposure to ethanol used for the WSP lines.

Each control line consists of five families that are bred to each other by rotation. The effect is to allow the genome to segregate randomly.

Used in conjunction with the Withdrawal Seizure-Prone, replicate 1 and 2 (WSP1, WSP2) and the Resistant lines (WSR1, WSR2) they may be useful for studying the genetic and neurobiological basis of alcohol withdrawal severity.

Development

Withdrawal Seizure-Control, replicate 1 (WSC1) mice were derived from the HS/Ibg heterogeneous stock that was developed by Gerald McClearn and colleagues (The Institute for Behavioral Genetics, Boulder, Colorado) as an 8-way cross of the following inbred strains obtained from the Cancer Research Genetics Laboratory at UC-Berkeley: A, AKR, BALB/c, C3H/2, C57BL, DBA/2, Is/Bi, and RIII.

Eighteen matings of Generation 30 HS/Ibg mice were randomly divided into 2 groups of 9 families each. These generation 31 mice became the foundation (S_o) population. One group of 9 families was designated Replicate 1, the other Replicate 2. Progeny from each family were then mated using a rotational system – female from Family 1 mated to male from Family 2, female from Family 2 mated to male from Family 3, etc. Two to six reserve families were generated to provide back up. Relaxed breeding (random selection of breeders from within each family) has continued for approximately 100 generations. The MMRRC imported a combination of the 9 families to form the WSC1 line in 2016.

Control Suggestions

Withdrawal Seizure Control Replicate 1 and 2 (WSC1, WSC2) lines serve as controls for the WSP1, WSP2, WSR1 and WSR1 lines.

Additional Information

Considerations for Choosing Controls

Selected References

Crabbe JC; Kosobud A; Young ER; Tam BR; McSwigan JD. 1985. Bidirectional selection for susceptibility to ethanol withdrawal seizures in Mus musculus. Behav Genet 15(6):521-36PubMed: 4096679 MGI: J:24807

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Genetics

Currently there are no related genes or alleles for this strain.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Gene Mapping: Tools for QTL Mapping, Segregation and Linkage Analysis
Neurobiology Research
- Behavioral and Learning Defects
  - genes regulating preferences to alcohol

Mammalian Phenotype Terms by Genotype

References

Crabbe JC; Kosobud A; Young ER; Tam BR; McSwigan JD. 1985. Bidirectional selection for susceptibility to ethanol withdrawal seizures in Mus musculus. Behav Genet 15(6):521-36PubMed: 4096679 MGI: J:24807

Additional References

Bergeson SE; Kyle Warren R; Crabbe JC; Metten P; Gene Erwin V; Belknap JK. 2003. Chromosomal loci influencing chronic alcohol withdrawal severity. Mamm Genome 14(7):454-63PubMed: 12925894 MGI: J:84090
Crabbe JC; Merrill CD; Belknap JK. 1991. Effects of convulsants on handling-induced convulsions in mice selected for ethanol withdrawal severity. Brain Res 550(1):1-6PubMed: 1888987 MGI: J:27582
Crabbe JC; Spence SE; Huang LC; Cameron AJ; Schlumbohm JP; Barkley-Levenson AM; Metten P. 2013. Ethanol drinking in withdrawal seizure-prone and -resistant selected mouse lines. Alcohol 47(5):381-9PubMed: 23809872 MGI: J:230485
Feller DJ; Bassir JM; Crabbe JC; Le Fevre CA. 1994. Audiogenic seizure susceptibility in WSP and WSR mice. Epilepsia 35(4):861-7PubMed: 8082635 MGI: J:20746
Hashimoto JG; Forquer MR; Tanchuck MA; Finn DA; Wiren KM. 2011. Importance of genetic background for risk of relapse shown in altered prefrontal cortex gene expression during abstinence following chronic alcohol intoxication. Neuroscience 173:57-75PubMed: 21081154 MGI: J:170269
Wilhelm CJ; Hashimoto JG; Roberts ML; Sonmez MK; Wiren KM. 2014. Understanding the addiction cycle: a complex biology with distinct contributions of genotype vs. sex at each stage. Neuroscience 279:168-86PubMed: 25194791 MGI: J:215897

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Breeding Considerations

The Withdrawal Seizure-Control, replicate 1 line consists of 5 families that were combined to form the WSC1 line. This line is maintained as a closed mating system.
- Additional Breeding and Husbandry Support
Mating System
- Sibling x Sibling
Citation
When using the Withdrawal Seizure Control, Replicate 1 mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #41489 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Overview

Also Known As:Withdrawal Seizure Control, Replicate 1

Donating Investigator

Genetic overview

Research Applications

Details

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Genetics

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional References

Technical Support

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Terms Of Use

Licensing Information

Related Strains