B6;129P2-Tspan3^{Gt(NPX312)Byg}/ReyaJ

Stock number: 028735
Product name: Tspan3 Gt KO
Research areas: Cancer Research, Hematological Research, Research Tools

Description

This Tspan3 (tetraspanin 3) gene trap strain is useful in studies of leukemogenesis, leukemia development and malignancy.

Detailed description

Tetraspanin 3 is encoded by the Tspan3 gene, contains 4 transmembrane domains, and is involved in oligodendrocyte migration, and signal transduction. Tspan3 is a downstream target of musashi 2 (Msi2). Overexpression of MSI2 is an indicator of poor prognosis in myeloid leukemias. These mice carry a gene trap knock-out allele of the Tspan3 gene. Mice that are homozygous for the targeted mutation are viable and fertile. Almost no gene product (mRNA) is detected by RT-PCR analysis of spleen and whole bone marrow cells from homozygotes. At 6 weeks of age, homozygotes exhibit total bone marrow cell numbers similar to wildtype controls. By 3 months of age, homozygotes exhibit hematopoietic abnormalities, including reduction (27% to 60%) of hematopoietic progenitors and differentiated cells. In serial tumor transplantation, homozygotes displayed a reduced incidence of leukemia compared to wildtype controls.

Development

A gene trap vector containing splice-acceptor sequence upstream of a β-geo (a fusion of β-galactosidase and neomycin phosphotransferase II) was inserted between exons 3 and 4. The construct was electroporated into unspecified 129P2/OlaHsd derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were bred to C57BL/6 mice to achieve germline transmission. Heterozygotes were intercrossed to generate homozygotes. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Tspan3^{Gt(NPX312)Byg}
Name: gene trap NPX312, BayGenomics
MGI accession ID: MGI:4125242
Generation method: Gene trapped
Attributes: Null/Knockout
Marker symbol: Tspan3
Marker name: tetraspanin 3
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Molecular note: The gene trap vector is inserted between exons 3 and 4.