This Tspan3 (tetraspanin 3) gene trap strain is useful in studies of leukemogenesis, leukemia development and malignancy.
Tannishtha Reya, University of California, San Diego
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Gene trapped (Null/Knockout) | Tspan3 | tetraspanin 3 |
Tetraspanin 3 is encoded by the Tspan3 gene, contains 4 transmembrane domains, and is involved in oligodendrocyte migration, and signal transduction.
Tspan3 is a downstream target of musashi 2 (Msi2). Overexpression of MSI2 is an indicator of poor prognosis in myeloid leukemias. These mice carry a gene trap knock-out allele of the Tspan3 gene. Mice that are homozygous for the targeted mutation are viable and fertile. Almost no gene product (mRNA) is detected by RT-PCR analysis of spleen and whole bone marrow cells from homozygotes. At 6 weeks of age, homozygotes exhibit total bone marrow cell numbers similar to wildtype controls. By 3 months of age, homozygotes exhibit hematopoietic abnormalities, including reduction (27% to 60%) of hematopoietic progenitors and differentiated cells. In serial tumor transplantation, homozygotes displayed a reduced incidence of leukemia compared to wildtype controls.
A gene trap vector containing splice-acceptor sequence upstream of a β-geo (a fusion of β-galactosidase and neomycin phosphotransferase II) was inserted between exons 3 and 4. The construct was electroporated into unspecified 129P2/OlaHsd derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.
The resulting chimeric animals were bred to C57BL/6 mice to achieve germline transmission.
Heterozygotes were intercrossed to generate homozygotes.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Allele Name | gene trap NPX312, BayGenomics |
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Allele Type | Gene trapped (Null/Knockout) |
Allele Synonym(s) | Tspan3 Gt` |
Gene Symbol and Name | Tspan3, tetraspanin 3 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | The gene trap vector is inserted between exons 3 and 4. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Tspan3 Gt KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #028735 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Tspan3<Gt(NPX312)Byg> |
Frozen Mouse Embryo | B6;129P2-Tspan3<Gt(NPX312)Byg>/ReyaJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Tspan3<Gt(NPX312)Byg>/ReyaJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Tspan3<Gt(NPX312)Byg>/ReyaJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Tspan3<Gt(NPX312)Byg>/ReyaJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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