Overview

When bred to mice expressing Cre recombinase, these transgenic mice express a Wnt1:GFP fusion protein in the cre-expressing tissues. This strain may be useful in studies of kidney development and organogenesis.

Donating Investigator

Andrew P McMahon, University of Southern California

Genetic overview

Genetic Background	Generation

Gt(ROSA)26Sor^{tm2(Wnt1/GFP)Amc}

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence)	Gt(ROSA)26Sor	gene trap ROSA 26, Philippe Soriano

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Research Applications

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

These knock-in mice carry a floxed STOP cassette and the Wnt1-GFP fusion gene inserted into the Gt(ROSA)26Sor locus. When bred to mice expressing Cre recombinase, the STOP sequence is deleted and Wnt1-GFP expression is observed in the cre-expressing tissue(s) of the double mutant offspring. These mutant mice may be useful in monitoring the Cre expression during embryonic development.

Mice that are homozygous for the targeted mutation are viable and fertile.

Development

A targeting vector containing a floxed triple polyadenylation
sequence and a full-length Wnt1 and GFP fusion gene was used to insert the floxed STOP cassette and the Wnt1-GFP fusion gene into the Gt(ROSA)26Sor locus. The Rosa26Wnt1EGFP construct was electroporated into 129X1/SvJ derived AV3 embryonic stem (ES) cells.
Correctly targeted ES cells were injected into blastocysts.
The resulting chimeric animals were tested for germline transmission.
The mice were then outcrossed to Swiss Webster and maintained on a mixed background of C57BL/6, 129X1/SvJ and Swiss Webster.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	GFP fusion protein is expressed in cre-expressing tissues when these mice are bred with mice carrying cre recombinase.

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Carroll TJ; Park JS; Hayashi S; Majumdar A; McMahon AP. 2005. Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. Dev Cell 9(2):283-92PubMed: 16054034 MGI: J:100575

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Genetics

Gt(ROSA)26Sor^{tm2(Wnt1/GFP)Amc}

Allele Symbol: Gt(ROSA)26Sor^{tm2(Wnt1/GFP)Amc}

Allele Name	targeted mutation 2, Andrew P McMahon
Allele Type	Targeted (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence)
Allele Synonym(s)	Gt(ROSA)26Sor^{tm2(Wnt1/GFP)Amc}; targeted mutation 2, Andrew P McMahon
Gene Symbol and Name	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Gene Synonym(s)	beta geo; ROSA26; R26; Gtrgeo26; Gtrosa26; Gtrgeo26; Gtrosa26; AV258896; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; SETD5-AS1; Thumpd3as1
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	GFP fusion protein is expressed in cre-expressing tissues when these mice are bred with mice carrying cre recombinase.
Strain of Origin	129X1/SvJ
Chromosome	6
Molecular Note	A full-length Wnt1-GFP construct was inserted into the locus downstream of a floxed triple polyA sequence. Upon cre expression, the cassette is removed and Wnt1-GFP is expressed.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Carroll TJ; Park JS; Hayashi S; Majumdar A; McMahon AP. 2005. Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. Dev Cell 9(2):283-92PubMed: 16054034 MGI: J:100575

Additional - Gt(ROSA)26Sor^{tm2(Wnt1/GFP)Amc} related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Gt(ROSA)26Sor^{tm2(Wnt1/GFP)Amc}
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes.
- Additional Breeding and Husbandry Support
Mating System
- Heterozygote x Heterozygote
Citation
When using the STOCK Gt(ROSA)26Sor^{tm2(Wnt1/GFP)Amc}/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #028733 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	heterozygous or wildtype for Gt(ROSA)26Sor<tm2(Wnt1/GFP)Amc>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	STOCK Gt(ROSA)26Sor<tm2(Wnt1/GFP)Amc>/J	$2595.00
Frozen Mouse Embryo	STOCK Gt(ROSA)26Sor<tm2(Wnt1/GFP)Amc>/J	$2595.00
Frozen Mouse Embryo	STOCK Gt(ROSA)26Sor<tm2(Wnt1/GFP)Amc>/J	$3373.50
Frozen Mouse Embryo	STOCK Gt(ROSA)26Sor<tm2(Wnt1/GFP)Amc>/J	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Gt(ROSA)26Sortm2(Wnt1/GFP)Amc

Allele Symbol: Gt(ROSA)26Sortm2(Wnt1/GFP)Amc

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Gt(ROSA)26Sortm2(Wnt1/GFP)Amc related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Gt(ROSA)26Sor^{tm2(Wnt1/GFP)Amc}

Allele Symbol: Gt(ROSA)26Sor^{tm2(Wnt1/GFP)Amc}

Additional - Gt(ROSA)26Sor^{tm2(Wnt1/GFP)Amc} related