When bred to mice expressing Cre recombinase, these transgenic mice express a Wnt1:GFP fusion protein in the cre-expressing tissues. This strain may be useful in studies of kidney development and organogenesis.
Andrew P McMahon, University of Southern California
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
These knock-in mice carry a floxed STOP cassette and the Wnt1-GFP fusion gene inserted into the Gt(ROSA)26Sor locus. When bred to mice expressing Cre recombinase, the STOP sequence is deleted and Wnt1-GFP expression is observed in the cre-expressing tissue(s) of the double mutant offspring. These mutant mice may be useful in monitoring the Cre expression during embryonic development.
Mice that are homozygous for the targeted mutation are viable and fertile.
A targeting vector containing a floxed triple polyadenylation
sequence and a full-length Wnt1 and GFP fusion gene was used to insert the floxed STOP cassette and the Wnt1-GFP fusion gene into the Gt(ROSA)26Sor locus. The Rosa26Wnt1EGFP construct was electroporated into 129X1/SvJ derived AV3 embryonic stem (ES) cells.
Correctly targeted ES cells were injected into blastocysts.
The resulting chimeric animals were tested for germline transmission.
The mice were then outcrossed to Swiss Webster and maintained on a mixed background of C57BL/6, 129X1/SvJ and Swiss Webster.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP fusion protein is expressed in cre-expressing tissues when these mice are bred with mice carrying cre recombinase. |
Allele Name | targeted mutation 2, Andrew P McMahon |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence) |
Allele Synonym(s) | ROSA-Wnt1 |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP fusion protein is expressed in cre-expressing tissues when these mice are bred with mice carrying cre recombinase. |
Strain of Origin | 129X1/SvJ |
Chromosome | 6 |
Molecular Note | A full-length Wnt1-GFP construct was inserted into the locus downstream of a floxed triple polyA sequence. Upon cre expression, the cassette is removed and Wnt1-GFP is expressed. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the STOCK Gt(ROSA)26Sortm2(Wnt1/GFP)Amc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #028733 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
heterozygous or wildtype for Gt(ROSA)26Sor<tm2(Wnt1/GFP)Amc> |
Frozen Mouse Embryo | STOCK Gt(ROSA)26Sor<tm2(Wnt1/GFP)Amc>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gt(ROSA)26Sor<tm2(Wnt1/GFP)Amc>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gt(ROSA)26Sor<tm2(Wnt1/GFP)Amc>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Gt(ROSA)26Sor<tm2(Wnt1/GFP)Amc>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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