STOCK Gt(ROSA)26Sor^{tm2(Wnt1/GFP)Amc}/J

Stock number: 028733

Description

When bred to mice expressing Cre recombinase, these transgenic mice express a Wnt1:GFP fusion protein in the cre-expressing tissues. This strain may be useful in studies of kidney development and organogenesis.

Detailed description

These knock-in mice carry a floxed STOP cassette and the Wnt1-GFP fusion gene inserted into the Gt(ROSA)26Sor locus. When bred to mice expressing Cre recombinase, the STOP sequence is deleted and Wnt1-GFP expression is observed in the cre-expressing tissue(s) of the double mutant offspring. These mutant mice may be useful in monitoring the Cre expression during embryonic development.

Mice that are homozygous for the targeted mutation are viable and fertile.

Development

A targeting vector containing a floxed triple polyadenylation sequence and a full-length Wnt1 and GFP fusion gene was used to insert the floxed STOP cassette and the Wnt1-GFP fusion gene into the Gt(ROSA)26Sor locus. The Rosa26Wnt1EGFP construct was electroporated into 129X1/SvJ derived AV3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were then outcrossed to Swiss Webster and maintained on a mixed background of C57BL/6, 129X1/SvJ and Swiss Webster. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Gt(ROSA)26Sor^{tm2(Wnt1/GFP)Amc}
Name: targeted mutation 2, Andrew P McMahon
MGI accession ID: MGI:3589804
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: 129X1/SvJ
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP fusion protein is expressed in cre-expressing tissues when these mice are bred with mice carrying cre recombinase.
Molecular note: A full-length Wnt1-GFP construct was inserted into the locus downstream of a floxed triple polyA sequence. Upon cre expression, the cassette is removed and Wnt1-GFP is expressed.