The NSE-hENT1 transgene contains a rat neuron-specific enolase (Eno2) promoter directing expression of a human solute carrier family 29 (nucleoside transporters), member 1 (SLC29A1). SLC29A1 or ENT1 mediates adenosine influx and efflux. Transgenic mice exhibit altered responses to caffeine, alcohol, hypoxia and ischemia.
Fiona Parkinson, University of Manitoba
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
The NSE-hENT1 transgene contains a rat neuron-specific enolase (Eno2) promoter directing expression of a human solute carrier family 29 (nucleoside transporters), member 1 (SLC29A1 or ENT1). Expression is detected in the cerebral cortex, striatum, hippocampus and cerebellum, but not in skeletal muscle, heart, liver, kidney and lung. SLC29A1 is an equilibrative nucleoside transporter that mediates adenosine influx and efflux. Cortical synaptosomes exhibit increased [3H]adenosine uptake and [3H]nitrobenzylthioinosine binding. In response to caffeine administration, transgenic mice exhibit peak activity levels similar to wild-type, however, within 60 minutes there is a more rapid decrease in horizontal activity and fine movements. In response to ethanol administration, the duration of LORR (loss of righting response) is enhanced as compared to wild-type. In addition, mice exhibit reduced synaptic depression following hypoxia/ischemia and enhanced stroke injury. Both homozygote and hemizygote mice exhibit similar phenotypes, however, in biochemistry and some behavior experiments (ligand binding, western blot, qRT-PCR, ethanol-induced loss of righting response) homozygotes exhibit a greater magnitude of response. This strain may be useful for studying drug sensitivity and stroke.
The NSE-hENT1 transgene contains a rat neuron-specific enolase (Eno2) promoter (including regulatory sequences: exon 1, intron 1, and 6 bp of exon 2) directing expression of a human solute carrier family 29 (nucleoside transporters), member 1 (SLC29A1 or ENT1) cDNA followed by a polyA signal. The SLC29A1 transcription initiation codon is in-frame with exon 2. The 6.4 kb transgenic construct was microinjected into the male pronuclei of CD1 oocytes, and mice from founder line 6928 were bred to CD1 mice to establish a colony. Mice were maintained by breeding to CD1 mice. Upon arrival, mice were bred to C57BL/6J for at least one generation to establish the colony.
Expressed Gene | SLC29A1, solute carrier family 29 member 1 (Augustine blood group), human |
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Site of Expression |
Allele Name | transgene insertion 6928, Fiona Parkinson |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | NSE-hENT1 |
Gene Symbol and Name | Tg(Eno2-SLC29A1)6928Fpar, transgene insertion 6928, Fiona Parkinson |
Gene Synonym(s) | |
Promoter | Eno2, enolase 2, gamma, neuronal, rat |
Expressed Gene | SLC29A1, solute carrier family 29 member 1 (Augustine blood group), human |
Strain of Origin | CD-1 |
Chromosome | UN |
Molecular Note | The transgene contains a rat neuron-specific enolase (Eno2) promoter (including regulatory sequences: exon 1, intron 1, and 6 bp of exon 2) directing expression of a human solute carrier family 29 (nucleoside transporters), member 1 (SLC29A1 or ENT1) cDNA followed by a polyA signal. The SLC29A1 transcription initiation codon is in-frame with exon 2. Expression is detected in the cerebral cortex, striatum, hippocampus and cerebellum. RNA samples from skeletal muscle, heart, liver, kidney and lung are negative for transgene expression. |
While maintaining a live colony, these mice are bred as homozygotes.
When using the NSE-hENT1 Tg mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #41849 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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