TβRIf/f floxed mice possess loxP sites flanking exon 3 of the Tgfbr1 gene making this strain useful for studying vascular development and hematopoiesis.
Stefan Karlsson, Lund University Hospital
TβRIf/f floxed mice possess loxP sites flanking exon 3 of the transforming growth factor, beta receptor I (Tgfbr1) gene. TβRI is a transmembrane serine/threonine kinase that forms a heteromeric complex with TβRII. TGF-β signaling through this complex is important for the regulation of vascular development, hematopoiesis, autoimmune diseases, and chronic inflammation. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.
A targeting vector was designed by Dr. Stefan Karlsson (Lund University) to insert a loxP-flanked neomycin resistance (neo) cassette site upstream of exon 3, and a third loxP site downstream of exon 3 of the transforming growth factor, beta receptor I (Tgfbr1) gene. The construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a pIC-Cre expression plasmid to delete the neo cassette. Resulting ES cells contained multiple gene rearrangements; intact floxed-exon 3, intact floxed-neo cassette, or excision of both exon 3 and the neo cassette. Correctly targeted ES cells, containing only the floxed-exon 3, were injected into C57BL/6 blastocysts. Resulting TβRIf/f offspring were bred to CD-1 mice and C57BL/6 mice and were maintained on a STOCK background. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1.1, Stefan Karlsson|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Tgfbr1tm1.1Karl; targeted mutation 1.1, Stefan Karlsson|
|Gene Symbol and Name||Tgfbr1, transforming growth factor, beta receptor I|
|Gene Synonym(s)||ALK5; ACVRLK4; MSSE; expressed sequence AU017191; AAT5; AU017191; LDS1A; LDS2A; ESS1; SKR4; ALK-5; TGFR-1; LDS1; TbetaR-I; TbetaRI; Skr4; Tgfr-1; Alk-5; tbetaR-I; Alk5; TBR-i; TBRI|
|Strain of Origin||129|
|General Note||ES cell line = R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl+) or GS (129S6/SvEvTac).|
|Molecular Note||Exon 3 was left flanked by single loxP sites after the removal of an upstream floxed neo cassette by the transient expression of cre recombinase in ES cells.|
When maintaining a live colony, homozygous mice may be bred together.
When using the TβRIf mouse strain in a publication, please cite the originating article(s) and include JAX stock #028701 in your Materials and Methods section.
|Heterozygous for Tgfbr1<tm1.1Karl>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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