These floxed mutant mice possess loxP sites flanking exons 3 through 10 of the Slc44a2 gene, also known as Ctl2. This strain may be useful for generating conditional mutations in applications related to cochlear hair cell survival and antibody-induced hearing loss.
Thomas E Carey, University of Michigan
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed)) | Slc44a2 | solute carrier family 44, member 2 |
These mice possess loxP sites flanking exons 3 through 10 of the targeted Slc44a2 gene. Solute carrier family 44, member 2, also known as choline transporter-like protein 2, encoded by the Slc44a2 gene, is a transmembrane glycoprotein that plays a role in auditory hair cell survival and is associated with antibody-induced hearing loss.
Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 through 10 deleted in the cre-expressing tissues.
Removal of the floxed sequence creates a null allele. The FVB/NJ genetic background confers resistance to age-related hearing loss.
When bred to a strain with germ line Cre recombinase expression (see Stock No. 003724 for example) knock-out mice will be produced, which may be useful in studies of cochlear hair cell survival and antibody-induced hearing loss.
A targeting vector containing a FRT site flanked PGK-Neo selection cassette followed by a loxP site was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 3 of the targeted gene, and another loxP site was inserted upstream of exon 10. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Resulting chimeric male animals were crossed to wildtype C57BL/6 mice. The Slc44a2neo mice were crossed to Tg(ACTFLPe)9205Dym transgenic mice expressing FLP recombinase under the control of the human ACTB promoter to remove the FRT site flanked NEO cassette. Mice that retained the loxP site flanked exons 3-10, and no longer contained the selection cassette, were then bred to FVB/NJ mice to remove the FLP allele/transgene. The mice were backcrossed to FVB/NJ for 7 generations. Homozygous male mice were crossed with female wildtype FVB/NJ mice to generate embryos for cryopreservation. Upon arrival, to establish our live colony, cryopreserved embryos were recovered.
Allele Name | targeted mutation 1.1, Thomas E Carey |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed)) |
Allele Synonym(s) | Slc44a2flox |
Gene Symbol and Name | Slc44a2, solute carrier family 44, member 2 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 9 |
Molecular Note | A targeting vector containing an FRT-flanked PGK-Neo selection cassette followed by a loxP site inserted upstream of exon 3 and another loxP site was inserted downstream of exon 10. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exons 3 through 10 floxed. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Slc44a2flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #028671 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Slc44a2<tm1.1Tec> |
Frozen Mouse Embryo | FVB.129(B6)-Slc44a2<tm1.1Tec>/J | $2595.00 |
Frozen Mouse Embryo | FVB.129(B6)-Slc44a2<tm1.1Tec>/J | $2595.00 |
Frozen Mouse Embryo | FVB.129(B6)-Slc44a2<tm1.1Tec>/J | $3373.50 |
Frozen Mouse Embryo | FVB.129(B6)-Slc44a2<tm1.1Tec>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.